|Other Names||Lymphocyte antigen 96, Ly-96, ESOP-1, Protein MD-2, LY96, ESOP1, MD2|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP6351a was selected from the N-term region of human MD2. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||The synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml deionized water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Binds bacterial lipopolysaccharide (LPS) (PubMed:17803912, PubMed:17569869). Cooperates with TLR4 in the innate immune response to bacterial lipopolysaccharide (LPS), and with TLR2 in the response to cell wall components from Gram- positive and Gram-negative bacteria (PubMed:11160242, PubMed:11593030). Enhances TLR4-dependent activation of NF-kappa-B (PubMed:10359581). Cells expressing both LY96 and TLR4, but not TLR4 alone, respond to LPS (PubMed:10359581).|
|Cellular Location||Secreted, extracellular space. Secreted. Note=Retained in the extracellular space at the cell surface by interaction with TLR4 (PubMed:10359581).|
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Provided below are standard protocols that you may find useful for product applications.
MD2 cooperates with toll-like receptor 4 (TLR4)on the cell surface in the innate immune response to bacterial lipopolysaccharide (LPS), and with TLR2 in the response to cell wall components from Gram-positive and Gram-negative bacteria. This protein enhances TLR4-dependent activation of NF-kappa-B. Cells expressing both MD2 and TLR4, but not TLR4 alone, respond to LPS.
Nishitani, C., et al., Biochem. Biophys. Res. Commun. 328(2):586-590 (2005).Pugin, J., et al., Blood 104(13):4071-4079 (2004).Hyakushima, N., et al., J. Immunol. 173(11):6949-6954 (2004).Fujimoto, T., et al., J. Biol. Chem. 279(46):47431-47437 (2004).Hamann, L., et al., Genes Immun. 5(4):283-288 (2004).
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