|Other Names||Dedicator of cytokinesis protein 1, 180 kDa protein downstream of CRK, DOCK180, DOCK1|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP6697b was selected from the C-term region of human DOCK1. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Along with DOCK1, mediates CRK/CRKL regulation of epithelial and endothelial cell spreading and migration on type IV collagen (PubMed:19004829). Functions as a guanine nucleotide exchange factor (GEF), which activates Rac Rho small GTPases by exchanging bound GDP for free GTP. Its GEF activity may be enhanced by ELMO1 (PubMed:8657152).|
|Cellular Location||Cytoplasm. Membrane. Note=Recruited to membranes via its interaction with phosphatidylinositol 3,4,5-trisphosphate.|
|Tissue Location||Highly expressed in placenta, lung, kidney, pancreas and ovary. Expressed at intermediate level in thymus, testes and colon|
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Provided below are standard protocols that you may find useful for product applications.
DOCK1 binds to the SH3 domain of CRK protein. It may regulate cell surface extension and may have a role in the cell surface extension of an engulfing cell around a dying cell during apoptosis.
Komander,D., Mol. Biol. Cell 19 (11), 4837-4851 (2008)Smith,H.W., J. Cell Biol. 182 (4), 777-790 (2008)
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