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MLF1 Antibody (N-term) Blocking Peptide
Synthetic peptide
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
| Primary Accession | P58340 |
|---|---|
| Clone Names | 80902027 |
| Gene ID | 4291 |
|---|---|
| Other Names | Myeloid leukemia factor 1, Myelodysplasia-myeloid leukemia factor 1, MLF1 |
| Target/Specificity | The synthetic peptide sequence used to generate the antibody AP6716a was selected from the N-term region of human MLF1. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay. |
| Format | Peptides are lyophilized in a solid powder format. Peptides can be reconstituted in solution using the appropriate buffer as needed. |
| Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C. |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Name | MLF1 |
|---|---|
| Function | Involved in lineage commitment of primary hemopoietic progenitors by restricting erythroid formation and enhancing myeloid formation. Interferes with erythropoietin-induced erythroid terminal differentiation by preventing cells from exiting the cell cycle through suppression of CDKN1B/p27Kip1 levels. Suppresses COP1 activity via CSN3 which activates p53 and induces cell cycle arrest. Binds DNA and affects the expression of a number of genes so may function as a transcription factor in the nucleus. |
| Cellular Location | Cytoplasm {ECO:0000250|UniProtKB:Q9QWV4}. Nucleus {ECO:0000250|UniProtKB:Q9QWV4}. Cell projection, cilium {ECO:0000250|UniProtKB:Q9QWV4}. Cytoplasm, cytoskeleton, cilium basal body {ECO:0000250|UniProtKB:Q9QWV4}. Note=Shuttles between the cytoplasm and nucleus. {ECO:0000250|UniProtKB:Q9QWV4} |
| Tissue Location | Most abundant in testis, ovary, skeletal muscle, heart, kidney and colon. Low expression in spleen, thymus and peripheral blood leukocytes |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
MLF1 is involved in lineage commitment of primary hemopoietic progenitors by restricting erythroid formation and enhancing myeloid formation. The protein interferes with erythopoietin-induced erythroid terminal differentiation by preventing cells from exiting the cell cycle through suppression of CDKN1B/p27Kip1 levels. It suppresses RFWD2/COP1 activity via CSN3 which activates p53 and induces cell cycle arrest. It binds DNA and affects the expression of a number of genes so may function as a transcription factor in the nucleus.
References
Li,Z.F., J. Neurol. Sci. 264 (1-2), 77-86 (2008)Yoneda-Kato,N., EMBO J. 24 (9), 1739-1749 (2005)
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