|Other Names||Mediator of RNA polymerase II transcription subunit 28, Endothelial-derived protein 1, Mediator complex subunit 28, Merlin and Grb2-interacting cytoskeletal protein, Magicin, Tumor angiogenesis marker EG-1, MED28, EG1|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP6873b was selected from the C-term region of human MED28. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. May be part of a complex containing NF2/merlin that participates in cellular signaling to the actin cytoskeleton downstream of tyrosine kinase signaling pathways.|
|Cellular Location||Nucleus. Cytoplasm. Membrane; Peripheral membrane protein. Note=According to PubMed:15467741, it is cytoplasmic and mainly membrane-associated|
|Tissue Location||Widely expressed. Highly expressed in vascular tissues such as placenta, testis and liver|
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Provided below are standard protocols that you may find useful for product applications.
MED28 may be part of a complex containing NF2/merlin that participates in cellular signaling to the actin cytoskeleton downstream of tyrosine kinase signaling pathways.
Lu,M., et.al., Cancer Res. 65 (14), 6159-6166 (2005)
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