|Other Names||Alpha-ketoglutarate-dependent dioxygenase FTO, 11411-, Fat mass and obesity-associated protein, FTO, KIAA1752|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP6976a was selected from the N-term region of human FTO. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Dioxygenase that repairs alkylated DNA and RNA by oxidative demethylation. Has highest activity towards single- stranded RNA containing 3-methyluracil, followed by single- stranded DNA containing 3-methylthymine. Has low demethylase activity towards single-stranded DNA containing 1-methyladenine or 3-methylcytosine (PubMed:18775698, PubMed:20376003). Specifically demethylates N(6)-methyladenosine (m6A) RNA, the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes (PubMed:22002720, PubMed:26458103). Has no activity towards 1- methylguanine. Has no detectable activity towards double-stranded DNA. Requires molecular oxygen, alpha-ketoglutarate and iron. Contributes to the regulation of the global metabolic rate, energy expenditure and energy homeostasis. Contributes to the regulation of body size and body fat accumulation (PubMed:18775698, PubMed:20376003). In particular, it is involved in the regulation of thermogenesis and the control of adipocyte differentiation into brown or white fat cells (PubMed:26287746).|
|Cellular Location||Nucleus. Nucleus speckle|
|Tissue Location||Ubiquitously expressed, with relatively high expression in adrenal glands and brain; especially in hypothalamus and pituitary.|
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Provided below are standard protocols that you may find useful for product applications.
The precise function of FTO remains to be determined.
Scott,L.J., et.al., Science 316 (5829), 1341-1345 (2007)
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