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CERK Antibody (N-term) Blocking Peptide

Synthetic peptide

     
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Product Information
Primary Accession Q8TCT0
Other Accession NP_073603
Clone Names 4010807
Additional Information
Other Names Ceramide kinase, hCERK, Acylsphingosine kinase, Lipid kinase 4, LK4, CERK, KIAA1646
Target/Specificity The synthetic peptide sequence used to generate the antibody AP7088a was selected from the N-term region of human CERK. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
Format The synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml deionized water for a final concentration of 1 mg/ml.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Protein Information
Name CERK
Synonyms KIAA1646
Function Catalyzes specifically the phosphorylation of ceramide to form ceramide 1-phosphate. Acts efficiently on natural and analog ceramides (C6, C8, C16 ceramides, and C8-dihydroceramide), to a lesser extent on C2-ceramide and C6-dihydroceramide, but not on other lipids, such as various sphingosines. Binds phosphoinositides.
Cellular Location Cytoplasm. Membrane; Peripheral membrane protein
Tissue Location High level expression in heart, brain, skeletal muscle, kidney and liver; moderate in peripheral blood leukocytes and thymus; very low in spleen, small intestine, placenta and lung EMBL; AB079066; BAC01154.1; -; mRNA EMBL; AJ457828; CAD29884.1; -; mRNA EMBL; CR456404; CAG30290.1; -; mRNA EMBL; AK291476; BAF84165.1; -; mRNA EMBL; AL096766; -; NOT_ANNOTATED_CDS; Genomic_DNA EMBL; AL118516; -; NOT_ANNOTATED_CDS; Genomic_DNA EMBL; CH471138; EAW73440.1; -; Genomic_DNA EMBL; CH471138; EAW73442.1; -; Genomic_DNA EMBL; BC067255; AAH67255.1; -; mRNA EMBL; BC126940; AAI26941.1; -; mRNA EMBL; AB051433; BAB33316.1; -; mRNA CCDS; CCDS14077.1; -. [Q8TCT0-1] RefSeq; NP_073603.2; NM_022766.5. [Q8TCT0-1] UniGene; Hs.200668; - ProteinModelPortal; Q8TCT0; - BioGrid; 122291; 48 IntAct; Q8TCT0; 47 STRING; 9606.ENSP00000216264; - BindingDB; Q8TCT0; - ChEMBL; CHEMBL1764936; - GuidetoPHARMACOLOGY; 2473; - SwissLipids; SLP:000001354; - iPTMnet; Q8TCT0; - PhosphoSitePlus; Q8TCT0; - BioMuta; CERK; - DMDM; 30172885; - EPD; Q8TCT0; - MaxQB; Q8TCT0; - PaxDb; Q8TCT0; - PeptideAtlas; Q8TCT0; - PRIDE; Q8TCT0; - ProteomicsDB; 74154; - DNASU; 64781; - Ensembl; ENST00000216264; ENSP00000216264; ENSG00000100422. [Q8TCT0-1] GeneID; 64781; - KEGG; hsa:64781; - UCSC; uc003bia.4; human. [Q8TCT0-1] CTD; 64781; - DisGeNET; 64781; - EuPathDB; HostDB:ENSG00000100422.13; - GeneCards; CERK; - HGNC; HGNC:19256; CERK HPA; HPA064699; - MIM; 610307; gene neXtProt; NX_Q8TCT0; - OpenTargets; ENSG00000100422; - PharmGKB; PA134958321; - eggNOG; KOG1115; Eukaryota eggNOG; COG1597; LUCA GeneTree; ENSGT00690000101761; - HOGENOM; HOG000111551; - HOVERGEN; HBG050903; - InParanoid; Q8TCT0; - KO; K04715; - OMA; NSSWNCD; - OrthoDB; EOG091G0FFW; - PhylomeDB; Q8TCT0; - TreeFam; TF314514; - BRENDA; 2.7.1.138; 2681 Reactome; R-HSA-1660662; Glycosphingolipid metabolism SABIO-RK; Q8TCT0; - ChiTaRS; CERK; human GeneWiki; CERK; - GenomeRNAi; 64781; - PRO; PR:Q8TCT0; - Proteomes; UP000005640; Chromosome 22 Bgee; ENSG00000100422; - CleanEx; HS_CERK; - ExpressionAtlas; Q8TCT0; baseline and differential Genevisible; Q8TCT0; HS GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell GO; GO:0016021; C:integral component of membrane; IDA:UniProtKB GO; GO:0005886; C:plasma membrane; TAS:Reactome GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW GO; GO:0001729; F:ceramide kinase activity; IDA:UniProtKB GO; GO:0102773; F:dihydroceramide kinase activity; IEA:UniProtKB-EC GO; GO:0000287; F:magnesium ion binding; IDA:UniProtKB GO; GO:0003951; F:NAD+ kinase activity; IEA:InterPro GO; GO:0006672; P:ceramide metabolic process; IDA:UniProtKB GO; GO:0006687; P:glycosphingolipid metabolic process; TAS:Reactome Gene3D; 3.40.50.10330; -; 1 InterPro; IPR017438; ATP-NAD_kinase_N InterPro; IPR001206; Diacylglycerol_kinase_cat_dom InterPro; IPR016064; NAD/diacylglycerol_kinase_sf Pfam; PF00781; DAGK_cat; 1 SMART; SM00046; DAGKc; 1 SUPFAM; SSF111331; SSF111331; 3 PROSITE; PS50146; DAGK; 1 1: Evidence at protein level; Alternative splicing; ATP-binding; Calcium; Complete proteome; Cytoplasm; Kinase; Magnesium; Membrane; Nucleotide-binding; Phosphoprotein; Polymorphism; Reference proteome; Transferase CHAIN 1 537 Ceramide kinase /FTId=PRO_0000181354 DOMAIN 128 278 DAGKc. {ECO:0000255|PROSITE- ProRule:PRU00783} NP_BIND 138 140 ATP. {ECO:0000255|PROSITE- ProRule:PRU00783} NP_BIND 170 174 ATP. {ECO:0000255|PROSITE- ProRule:PRU00783} NP_BIND 239 241 ATP. {ECO:0000255|PROSITE- ProRule:PRU00783} NP_BIND 502 504 ATP. {ECO:0000255|PROSITE- ProRule:PRU00783} REGION 1 125 Required for binding to sulfatide and phosphoinositides REGION 195 198 Substrate binding. ACT_SITE 197 197 Proton donor/acceptor. BINDING 202 202 ATP. {ECO:0000255|PROSITE- ProRule:PRU00783} BINDING 304 304 ATP. {ECO:0000255|PROSITE- ProRule:PRU00783} BINDING 310 310 ATP. {ECO:0000255|PROSITE- ProRule:PRU00783} MOD_RES 340 340 Phosphoserine MOD_RES 408 408 Phosphoserine VAR_SEQ 1 198 Missing (in isoform 2) /FTId=VSP_056944 VARIANT 191 191 I -> V (in dbSNP:rs16995615) /FTId=VAR_053685 VARIANT 211 211 T -> M (in dbSNP:rs9306515) /FTId=VAR_053686 VARIANT 306 306 L -> F (in dbSNP:rs13057352) /FTId=VAR_053687 MUTAGEN 10 10 L->A: 99% decrease in catalytic activity but no effect on substrate affinity MUTAGEN 10 10 L->I: 71% decrease in catalytic activity but no effect on substrate affinity MUTAGEN 340 340 S->A: 15% decrease in catalytic activity and decreased stability SEQUENCE 537 AA; 59977 MW; 3DBFC0ED8D679F7F CRC64; MGATGAAEPL QSVLWVKQQR CAVSLEPARA LLRWWRSPGP GAGAPGADAC SVPVSEIIAV EETDVHGKHQ GSGKWQKMEK PYAFTVHCVK RARRHRWKWA QVTFWCPEEQ LCHLWLQTLR EMLEKLTSRP KHLLVFINPF GGKGQGKRIY ERKVAPLFTL ASITTDIIVT EHANQAKETL YEINIDKYDG IVCVGGDGMF SEVLHGLIGR TQRSAGVDQN HPRAVLVPSS LRIGIIPAGS TDCVCYSTVG TSDAETSALH IVVGDSLAMD VSSVHHNSTL LRYSVSLLGY GFYGDIIKDS EKKRWLGLAR YDFSGLKTFL SHHCYEGTVS FLPAQHTVGS PRDRKPCRAG CFVCRQSKQQ LEEEQKKALY GLEAAEDVEE WQVVCGKFLA INATNMSCAC RRSPRGLSPA AHLGDGSSDL ILIRKCSRFN FLRFLIRHTN QQDQFDFTFV EVYRVKKFQF TSKHMEDEDS DLKEGGKKRF GHICSSHPSC CCTVSNSSWN CDGEVLHSPA IEVRVHCQLV RLFARGIEEN PKPDSHS
Research Areas
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Background

Ceramide kinases convert the sphingolipid metabolite ceramide into ceramide-1-phosphate, both key mediators of cellular apoptosis and survival. Ceramide metabolism plays an essential role in the viability of neuronal cells, the membranes of which are particularly rich in sphingolipids. CERK catalyzes specifically the phosphorylation of ceramide to form ceramide 1-phosphate. This enzyme acts efficiently on natural and analog ceramides (C6, C8, C16 ceramides, and C8 dihydroceramide), and to a lesser extent on C2-ceramide and C6-dihydroceramide, but not on other lipids, such as various sphingosines. High level expression is noted in heart, brain, skeletal muscle, kidney and liver; moderate expression in peripheral blood leukocytes and thymus; and low expression in spleen, small intestine, placenta and lung.

References

J. Biol. Chem. 279 (17), 17570-17577 (2004)J. Biol. Chem. 278 (40), 38206-38213 (2003)J. Biol. Chem. 277 (26), 23294-23300 (2002)

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$ 80.00
Cat# BP7088a
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