|Other Names||Alpha-protein kinase 1, 2711-, Chromosome 4 kinase, Lymphocyte alpha-protein kinase, ALPK1, KIAA1527, LAK|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP7109a was selected from the N-term region of human ALPK1. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||The synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml deionized water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Kinase that recognizes phosphorylation sites in which the surrounding peptides have an alpha-helical conformation.|
|Tissue Location||Highly expressed in liver.|
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Provided below are standard protocols that you may find useful for product applications.
Unlike most eukaryotic kinases, alpha kinases, such as ALPK1, recognize phosphorylation sites in which the surrounding peptides have an alpha-helical conformation. Epithelial cells maintain a polarized structure based on a selective sorting machinery for cargo traveling to the apical or the basolateral membrane domain at the trans-Golgi network exit. Alpha-kinase 1 (ALPK1)is a compnent of raft-carrying apical vesicles, originally identified in vesicles ferrying raft-associated sucrase-isomaltase (SI). It has been proposed that phosphorylation of myosin I by ALPK1 is essential to the apical trafficking of raft-associated SI.
Heine, M., et al., J. Biol. Chem. 280(27):25637-25643 (2005).Yamada, S., et al., Oncogene 23(35):5901-5911 (2004).Ryazanova, L.V., et al., Mol. Biol. (N.Y.) 35, 271-283 (2001) (): ().Ryazanov, A.G., et al., Curr. Biol. 9 (2), R43-R45 (1999) (): ().
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