|Other Names||NEDD8-activating enzyme E1 regulatory subunit, Amyloid beta precursor protein-binding protein 1, 59 kDa, APP-BP1, Amyloid protein-binding protein 1, Proto-oncogene protein 1, NAE1, APPBP1|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP7273b was selected from the C-term region of human APPBP1. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Regulatory subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Necessary for cell cycle progression through the S-M checkpoint. Overexpression of NAE1 causes apoptosis through deregulation of NEDD8 conjugation.|
|Cellular Location||Cell membrane. Note=Colocalizes with APP in lipid rafts|
|Tissue Location||Ubiquitous in fetal tissues. Expressed throughout the adult brain.|
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Provided below are standard protocols that you may find useful for product applications.
APPBP1 binds to the beta-amyloid precursor protein, a cell surface protein with signal-transducing properties thought to play a role in the pathogenesis of Alzheimer's disease. In addition, this protein can form a heterodimer with UBE1C and bind and activate NEDD8, a ubiquitin-like protein. APPB1 is required for cell cycle progression through the S/M checkpoint.
Chen,Y., J. Cell Biol. 163 (1), 27-33 (2003)Chen,Y., J. Neurochem. 85 (3), 801-809 (2003)Walden,H., Nature 422 (6929), 330-334 (2003)Chow,N., J. Biol. Chem. 271 (19), 11339-11346 (1996)
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