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CDK1 Antibody (T14) Blocking PeptideSynthetic peptide
| Country | United States
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| Catalog # | Size | Availability | Price | |
| BP7517d | 0.1 mg 400 ul | In Stock | $ 45.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
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- Backgrounds
CDK1 Antibody (T14) Blocking Peptide - Product info | |
| Primary Accession | P06493 |
| Clone Names | 5012152 |
| Calculated MW | 34095 Da |
CDK1 Antibody (T14) Blocking Peptide - Additional info | |
| Gene ID 983 | |
| Target/Specificity The synthetic peptide sequence used to generate the antibody AP7517d was selected from the T14 region of human CDK1. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay. | |
| Format Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C. | |
| Precautions This product is for research use only. Not for use in diagnostic or therapeutic procedures. | |
CDK1 Antibody (T14) Blocking Peptide - Protein Information | |
| Name CDK1 | |
| Synonyms CDC2, CDC28A, CDKN1, P34CDC2 | |
| Function Plays a key role in the control of the eukaryotic cell cycle by modulating the centrosome cycle as well as mitotic onset; promotes G2-M transition, and regulates G1 progress and G1-S transition via association with multiple interphase cyclins Required in higher cells for entry into S-phase and mitosis Phosphorylates PARVA/actopaxin, APC, AMPH, APC, BARD1, Bcl- xL/BCL2L1, BRCA2, CALD1, CASP8, CDC7, CDC20, CDC25A, CDC25C, CC2D1A, CSNK2 proteins/CKII, FZR1/CDH1, CDK7, CEBPB, CHAMP1, DMD/dystrophin, EEF1 proteins/EF-1, EZH2, KIF11/EG5, EGFR, FANCG, FOS, GFAP, GOLGA2/GM130, GRASP1, UBE2A/hHR6A, HIST1H1 proteins/histone H1, HMGA1, HIVEP3/KRC, LMNA, LMNB, LMNC, LBR, LATS1, MAP1B, MAP4, MARCKS, MCM2, MCM4, MKLP1, MYB, NEFH, NFIC, NPC/nuclear pore complex, PITPNM1/NIR2, NPM1, NCL, NUCKS1, NPM1/numatrin, ORC1, PRKAR2A, EEF1E1/p18, EIF3F/p47, p53/TP53, NONO/p54NRB, PAPOLA, PLEC/plectin, RB1, UL40/R2, RAB4A, RAP1GAP, RCC1, RPS6KB1/S6K1, KHDRBS1/SAM68, ESPL1, SKI, BIRC5/survivin, STIP1, TEX14, beta-tubulins, MAPT/TAU, NEDD1, VIM/vimentin, TK1, FOXO1, RUNX1/AML1 and RUNX2. CDK1/CDC2-cyclin-B controls pronuclear union in interphase fertilized eggs. Essential for early stages of embryonic development. During G2 and early mitosis, CDC25A/B/C-mediated dephosphorylation activates CDK1/cyclin complexes which phosphorylate several substrates that trigger at least centrosome separation, Golgi dynamics, nuclear envelope breakdown and chromosome condensation. Once chromosomes are condensed and aligned at the metaphase plate, CDK1 activity is switched off by WEE1- and PKMYT1-mediated phosphorylation to allow sister chromatid separation, chromosome decondensation, reformation of the nuclear envelope and cytokinesis. Inactivated by PKR/EIF2AK2- and WEE1-mediated phosphorylation upon DNA damage to stop cell cycle and genome replication at the G2 checkpoint thus facilitating DNA repair. Reactivated after successful DNA repair through WIP1-dependent signaling leading to CDC25A/B/C- mediated dephosphorylation and restoring cell cycle progression In proliferating cells, CDK1-mediated FOXO1 phosphorylation at the G2-M phase represses FOXO1 interaction with 14-3-3 proteins and thereby promotes FOXO1 nuclear accumulation and transcription factor activity, leading to cell death of postmitotic neurons. The phosphorylation of beta-tubulins regulates microtubule dynamics during mitosis. NEDD1 phosphorylation promotes PLK1-mediated NEDD1 phosphorylation and subsequent targeting of the gamma-tubulin ring complex (gTuRC) to the centrosome, an important step for spindle formation. In addition, CC2D1A phosphorylation regulates CC2D1A spindle pole localization and association with SCC1/RAD21 and centriole cohesion during mitosis. The phosphorylation of Bcl- xL/BCL2L1 after prolongated G2 arrest upon DNA damage triggers apoptosis. In contrast, CASP8 phosphorylation during mitosis prevents its activation by proteolysis and subsequent apoptosis This phosphorylation occurs in cancer cell lines, as well as in primary breast tissues and lymphocytes. EZH2 phosphorylation promotes H3K27me3 maintenance and epigenetic gene silencing. CALD1 phosphorylation promotes Schwann cell migration during peripheral nerve regeneration | |
| Cellular Location Nucleus. Cytoplasm. Mitochondrion. Cytoplasm, cytoskeleton, centrosome. Note=Cytoplasmic during the interphase. Reversibly translocated from cytoplasm to nucleus when phosphorylated before G2-M transition when associated with cyclin- B1. Accumulates in mitochondria in G2-arrested cells upon DNA- damage | |
| Tissue Location Isoform 2 is found in breast cancer tissues. | |
CDK1 Antibody (T14) Blocking Peptide - Related products
AP1497a: CDC2 Antibody (N-term)
AP1497b: CDC2 Antibody (C-term)
AP3056a: Phospho-CDC2-T161 Antibody
AP3059a: Phospho-CDK1-S39 Antibody
AP3482a: Phospho-CDK1-T14 Antibody
AP7517b: CDC2 (CDK1) Antibody (C-term)
AP7517c: CDK1 Antibody (Center K200)
BP1497a: CDC2 Antibody (N-term) Blocking Peptide
BP1497b: CDC2 Antibody (C-term) Blocking Peptide
BP3056a: Phospho-CDC2-T161 Blocking Peptide
BP3059a: Phospho-CDK1-S39 Antibody Blocking Peptide
BP3482a: Phospho-CDK1-T14 Antibody Blocking Peptide
BP7517b: CDK1 Antibody (C-term) Blocking Peptide
BP7517c: CDK1 Antibody (Center K200) Blocking Peptide
BP7517d: CDK1 Antibody (T14) Blocking Peptide
BP7517e: CDK1 Antibdoy (Y15) Blocking Peptide
CDK1 Antibody (T14) Blocking Peptide - Research Areas
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Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
CDK1 is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control.
REFERENCES
Kramer, A., et al., Nat. Cell Biol. 6(9):884-891 (2004).Dai, X., et al., J. Invest. Dermatol. 122(6):1356-1364 (2004).Litvak, V., et al., Mol. Cell 14(3):319-330 (2004).Shapira, M., et al., Cancer 100(8):1615-1621 (2004).Chow, J.P., et al., J. Biol. Chem. 278(42):40815-40828 (2003).
