|Other Names||Probable glutamate--tRNA ligase, mitochondrial, Glutamyl-tRNA synthetase, GluRS, EARS2, KIAA1970|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP7573b was selected from the C-term region of human EARS2. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).|
|Cellular Location||Mitochondrion matrix.|
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Provided below are standard protocols that you may find useful for product applications.
Glutamyl-tRNA synthetase (GluRS or EARS2) a class I aminoacyl-tRNA synthetase (aaRS), is primarily responsible for the glutamylation of tRNAGlu. It is part of the ??inimal set??of seventeen aaRSs found in every living organism and its presence is essential for the viability of cells.
Bonnefond,L.,Biochemistry 44 (12), 4805-4816 (2005)Daniel Y. Dubois, Jacques Lapointe and Shun-ichi Sekine, in Aminoacyl-tRNA Synthetases, Michael Ibba, ed (2005).
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