|Other Names||Probable histidine--tRNA ligase, mitochondrial, Histidine--tRNA ligase-like, Histidyl-tRNA synthetase, HisRS, HARS2, HARSL, HARSR, HO3|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP7584a was selected from the N-term region of human HARS2. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Synonyms||HARSL, HARSR, HO3|
|Cellular Location||Mitochondrion matrix.|
|Tissue Location||A high level expression is seen in the heart, kidney and skeletal muscle while a lower level expression is seen in the brain and liver|
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Provided below are standard protocols that you may find useful for product applications.
HARS2 is an enzyme belonging to the class II family of aminoacyl-tRNA synthetases. Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. Functioning in the synthesis of histidyl-transfer RNA, this enzyme plays an accessory role in the regulation of protein biosynthesis.
Freist,W., Biol. Chem. 380 (6), 623-646 (1999)O'Hanlon,T.P., Biochem. Biophys. Res. Commun. 210 (2), 556-566 (1995)Tsui,H.W.,Gene 131 (2), 201-208 (1993)
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