|Other Names||NGFI-A-binding protein 2, EGR-1-binding protein 2, Melanoma-associated delayed early response protein, Protein MADER, NAB2, MADER|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP7635c was selected from the Center region of human NAB2. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Acts as a transcriptional repressor for zinc finger transcription factors EGR1 and EGR2. Isoform 2 lacks repression ability (By similarity).|
|Cellular Location||Nucleus. Note=Isoform 2 is not localized to the nucleus.|
|Tissue Location||Widely expressed at low levels. Highly expressed in melanoma cell lines|
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Provided below are standard protocols that you may find useful for product applications.
NAB2 is a member of the family of NGFI-A binding (NAB) proteins, which function in the nucleus to repress transcription induced by some members of the EGR (early growth response) family of transactivators. NAB proteins can homo- or hetero-multimerize with other EGR or NAB proteins through a conserved N-terminal domain, and repress transcription through two partially redundant C-terminal domains. Transcriptional repression by this protein is mediated in part by interactions with the nucleosome remodeling and deactylase (NuRD) complex.
Srinivasan,R., J. Biol. Chem. 281 (22), 15129-15137 (2006)Kumbrink,J., J. Biol. Chem. 280 (52), 42785-42793 (2005)Lucerna,M., J. Biol. Chem. 278 (13), 11433-11440 (2003)
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