|Other Names||Pumilio homolog 1, HsPUM, Pumilio-1, PUM1, KIAA0099, PUMH1|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP7734b was selected from the C-term region of human PUM2. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Sequence-specific RNA-binding protein that negatively regulates translation and mRNA stability by binding the 3'-UTR of mRNA targets. Binds to an RNA consensus sequence, the Pumilio Response Element (PRE), 5'-UGUANAUA-3', that is related to the Nanos Response Element (NRE). Capable of deadenylation-dependent and -independent modes of repression. May be required to support proliferation and self-renewal of stem cells.|
|Tissue Location||Expressed in brain, heart, kidney, muscle, intestine and stomach. Not expressed in cerebellum, corpus callosum, caudate nucleus, hippocampus, medulla oblongata and putamen. Expressed in all fetal tissues tested|
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Provided below are standard protocols that you may find useful for product applications.
PUM2 is a sequence-specific RNA-binding protein that regulates translation and mRNA stability by binding the 3'-UTR of mRNA targets. Its interactions and tissue specificity suggest that it may be required to support proliferation and self-renewal of stem cells by regulating the translation of key transcripts.
Kusz,K., Mol. Reprod. Dev. 74 (6), 795-799 (2007)Spik,A., Reprod Biol 6 SUPPL 1, 37-42 (2006)Moore,F.L., Proc. Natl. Acad. Sci. U.S.A. 100 (2), 538-543 (2003)
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