|Other Names||Biotinidase, Biotinase, BTD|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP7752a was selected from the N-term region of human BTD. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Catalytic release of biotin from biocytin, the product of biotin-dependent carboxylases degradation.|
|Cellular Location||Secreted, extracellular space.|
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Provided below are standard protocols that you may find useful for product applications.
Biotinidase (BTD) functions to recycle biotin in the body by cleaving biocytin (biotin-epsilon-lysine), a normal product of carboxylase degradation, resulting in regeneration of free biotin. Biotinidase has also been shown to have biotinyl-transferase activity. Defects in the biotinidase gene cause multiple carboxylase deficiency.
Milankovics,I., Mol. Genet. Metab. 90 (3), 345-348 (2007)Wolf,B., Hum. Mutat. 25 (4), 413 (2005)
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