|Other Names||Probable proline--tRNA ligase, mitochondrial, Prolyl-tRNA synthetase, ProRS, PARS2|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP7782c was selected from the Center region of human PARS2. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Cellular Location||Mitochondrion matrix.|
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Provided below are standard protocols that you may find useful for product applications.
PARS2 catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro).
Bonnefond,L., Biochemistry 44 (12), 4805-4816 (2005)
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