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ZAP70 Antibody (Center) Blocking PeptideSynthetic peptide
| Country | United States
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| Catalog # | Size | Availability | Price | |
| BP7824c | 0.1 mg 400 ul | In Stock | $ 45.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
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ZAP70 Antibody (Center) Blocking Peptide - Product info | |
| Primary Accession | P43403 |
| Clone Names | 2123005 |
| Calculated MW | 69872 Da |
ZAP70 Antibody (Center) Blocking Peptide - Additional info | |
| Gene ID 7535 | |
| Target/Specificity The synthetic peptide sequence used to generate the antibody AP7824c was selected from the Center region of human ZAP70 . A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay. | |
| Format The synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml deionized water for a final concentration of 1 mg/ml. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C. | |
| Precautions This product is for research use only. Not for use in diagnostic or therapeutic procedures. | |
ZAP70 Antibody (Center) Blocking Peptide - Protein Information | |
| Name ZAP70 | |
| Synonyms SRK | |
| Function Tyrosine kinase that plays an essential role in regulation of the adaptive immune response. Regulates motility, adhesion and cytokine expression of mature T-cells, as well as thymocyte development. Contributes also to the development and activation of primary B-lymphocytes. When antigen presenting cells (APC) activate T-cell receptor (TCR), a serie of phosphorylations lead to the recruitment of ZAP70 to the doubly phosphorylated TCR component CD247/CD3Z through ITAM motif at the plasma membrane This recruitment serves to localization to the stimulated TCR and to relieve its autoinhibited conformation. Release of ZAP70 active conformation is further stabilized by phosphorylation mediated by LCK. Subsequently, ZAP70 phosphorylates at least 2 essential adapter proteins: LAT and LCP2. In turn, a large number of signaling molecules are recruited and ultimately lead to lymphokine production, T-cell proliferation and differentiation Furthermore, ZAP70 controls cytoskeleton modifications, adhesion and mobility of T-lymphocytes, thus ensuring correct delivery of effectors to the APC. ZAP70 is also required for TCR-CD247/CD3Z internalization and degradation through interaction with the E3 ubiquitin-protein ligase CBL and adapter proteins SLA and SLA2 Thus, ZAP70 regulates both T-cell activation switch on and switch off by modulating TCR expression at the T-cell surface. During thymocyte development, ZAP70 promotes survival and cell-cycle progression of developing thymocytes before positive selection (when cells are still CD4/CD8 double negative). Additionally, ZAP70-dependent signaling pathway may also contribute to primary B-cells formation and activation through B-cell receptor (BCR) | |
| Cellular Location Cytoplasm. Cell membrane; Peripheral membrane protein. Note=In quiescent T-lymphocytes, it is cytoplasmic. Upon TCR activation, it is recruited at the plasma membrane by interacting with CD247/CD3Z. Co-localizes together with RHOH in the immunological synapse. RHOH is required for its proper localization to the cell membrane and cytoskeleton fractions in the thymocytes (By similarity) | |
| Tissue Location Expressed in T- and natural killer cells. Also present in early thymocytes and pro/pre B-cells | |
ZAP70 Antibody (Center) Blocking Peptide - Related products
AP3676a: Phospho-ZAP70-Y292 Antibody
AP7824c: ZAP70 Antibody (Center)
RI15782: ZAP70 predesign siRNA
BP3658a: Phospho-ZAP70-Y319 Blocking Peptide
BP3659a: Phospho-ZAP70-Y492 Blocking Peptide
BP3660a: Phospho-ZAP70-Y493 Blocking Peptide
BP3661a: Phospho-ErbB2-pY1248(M) Blocking Peptide
BP3676a: Phospho-ZAP70-Y292 Antibody Blocking Peptide
BP7824c: ZAP70 Antibody (Center) Blocking Peptide
AJ1824b: ZAP-70 Antibody (C-term)
ZAP70 Antibody (Center) Blocking Peptide - Research Areas
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BACKGROUND
This gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene.
REFERENCES
Kuroyama, H., et al., Biochem. Biophys. Res. Commun. 315(4):935-941 (2004).Yang, Y., et al., Mol. Cell. Biol. 23(21):7667-7677 (2003).Kuhne, M.R., et al., J. Immunol. 171(2):860-866 (2003).Kremer, K.N., et al., J. Immunol. 171(1):360-367 (2003).Wiestner, A., et al., Blood 101(12):4944-4951 (2003).
