|Other Names||Methionine--tRNA ligase, mitochondrial, Methionyl-tRNA synthetase 2, Mitochondrial methionyl-tRNA synthetase, MtMetRS, MARS2|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP7841b was selected from the C-term region of human MARS2. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Cellular Location||Mitochondrion matrix.|
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Provided below are standard protocols that you may find useful for product applications.
Methionine-tRNA ligase (EC 22.214.171.124) catalyzes the chemical reaction: ATP + L-methionine + tRNAMet <--> AMP + diphosphate + L-methionyl-tRNAMet.This enzyme participates in three metabolic pathways: methionine metabolism, selenoamino acid metabolism, and aminoacyl-tRNA biosynthesis.
Spencer,A.C., Biochemistry 43 (30), 9743-9754 (2004)
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