|Other Names||Glucokinase regulatory protein, GKRP, Glucokinase regulator, GCKR|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP8143b was selected from the C-term region of human GCKR . A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||The synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml deionized water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Inhibits glucokinase (GCK) by forming an inactive complex with this enzyme. The affinity of GCKR for GCK is modulated by fructose metabolites: GCKR with bound fructose 6- phosphate has increased affinity for GCK, while GCKR with bound fructose 1-phosphate has strongly decreased affinity for GCK and does not inhibit GCK activity.|
|Cellular Location||Cytoplasm. Nucleus. Note=Under low glucose concentrations, GKRP associates with GCK and the inactive complex is recruited to the hepatocyte nucleus|
|Tissue Location||Found in liver and pancreas. Not detected in muscle, brain, heart, thymus, intestine, uterus, adipose tissue, kidney, adrenal, lung or spleen.|
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Provided below are standard protocols that you may find useful for product applications.
GCKR belongs to the SIS (Sugar ISomerase) family of proteins. The gene product is a regulatory protein that inhibits glucokinase in liver and pancreatic islet cells by binding non-covalently to form an inactive complex with the enzyme. Th GCKR gene is considered a susceptibility gene candidate for a form of maturity-onset diabetes of the young (MODY).
Veiga-da-Cunha, M., et al., Diabetologia 46(5):704-711 (2003).Hayward, B.E., et al., Genomics 49(1):137-142 (1998).Hayward, B.E., et al., Mamm. Genome 7(6):454-458 (1996).Warner, J.P., et al., Mamm. Genome 6(8):532-536 (1995).Vaxillaire, M., et al., Diabetes 43(3):389-395 (1994).
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