|Other Names||Glucokinase regulatory protein, GKRP, Glucokinase regulator, GCKR|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP8143b was selected from the C-term region of human GCKR . A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||The synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml deionized water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Inhibits glucokinase (GCK) by forming an inactive complex with this enzyme. The affinity of GCKR for GCK is modulated by fructose metabolites: GCKR with bound fructose 6- phosphate has increased affinity for GCK, while GCKR with bound fructose 1-phosphate has strongly decreased affinity for GCK and does not inhibit GCK activity.|
|Cellular Location||Cytoplasm. Nucleus. Note=Under low glucose concentrations, GKRP associates with GCK and the inactive complex is recruited to the hepatocyte nucleus|
|Tissue Location||Found in liver and pancreas. Not detected in muscle, brain, heart, thymus, intestine, uterus, adipose tissue, kidney, adrenal, lung or spleen.|
email@example.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
GCKR belongs to the SIS (Sugar ISomerase) family of proteins. The gene product is a regulatory protein that inhibits glucokinase in liver and pancreatic islet cells by binding non-covalently to form an inactive complex with the enzyme. Th GCKR gene is considered a susceptibility gene candidate for a form of maturity-onset diabetes of the young (MODY).
Veiga-da-Cunha, M., et al., Diabetologia 46(5):704-711 (2003).Hayward, B.E., et al., Genomics 49(1):137-142 (1998).Hayward, B.E., et al., Mamm. Genome 7(6):454-458 (1996).Warner, J.P., et al., Mamm. Genome 6(8):532-536 (1995).Vaxillaire, M., et al., Diabetes 43(3):389-395 (1994).
If you have any additional inquiries please email technical services at firstname.lastname@example.org.