SMPD1 Antibody (C-term) Blocking Peptide
Synthetic peptide
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Primary Accession | P17405 |
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Clone Names | 90625026 |
Gene ID | 6609 |
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Other Names | Sphingomyelin phosphodiesterase, Acid sphingomyelinase, aSMase, SMPD1, ASM |
Target/Specificity | The synthetic peptide sequence used to generate the antibody AP8882b was selected from the C-term region of human SMPD1. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay. |
Format | Peptides are lyophilized in a solid powder format. Peptides can be reconstituted in solution using the appropriate buffer as needed. |
Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C. |
Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
Name | SMPD1 (HGNC:11120) |
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Function | Converts sphingomyelin to ceramide (PubMed:1840600, PubMed:18815062, PubMed:27659707, PubMed:25920558, PubMed:25339683, PubMed:33163980, PubMed:12563314). Exists as two enzymatic forms that arise from alternative trafficking of a single protein precursor, one that is targeted to the endolysosomal compartment, whereas the other is released extracellularly (PubMed:21098024, PubMed:9660788, PubMed:20807762). However, in response to various forms of stress, lysosomal exocytosis may represent a major source of the secretory form (PubMed:20530211, PubMed:12563314, PubMed:20807762, PubMed:9393854, PubMed:22573858). |
Cellular Location | Lysosome. Lipid droplet. Secreted. Note=The secreted form is induced in a time- and dose-dependent by IL1B and TNF as well as stress and viral infection. This increase of the secreted form seems to be due to exocytosis of the lysosomal form and is Ca(2+)-dependent (PubMed:20807762, PubMed:22573858, PubMed:20530211). Secretion is dependent of phosphorylation at Ser-510 (PubMed:17303575). Secretion is induced by inflammatory mediators such as IL1B, IFNG or TNF as well as infection with bacteria and viruses (PubMed:12563314, PubMed:20807762) |
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Provided below are standard protocols that you may find useful for product applications.
Background
SMPD1 is a lysosomal acid sphingomyelinase that converts sphingomyelin to ceramide. This protein also has phospholipase C activity.
References
Takahashi,T., et.al., Hum. Mutat. 1 (1), 70-71 (1992)
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