|Other Names||Bile acyl-CoA synthetase, BACS, Bile acid-CoA ligase, BA-CoA ligase, BAL, Cholate--CoA ligase, Fatty acid transport protein 5, FATP-5, Fatty-acid-coenzyme A ligase, very long-chain 3, Solute carrier family 27 member 5, Very long-chain acyl-CoA synthetase homolog 2, VLCS-H2, VLCSH2, Very long-chain acyl-CoA synthetase-related protein, VLACS-related, VLACSR, SLC27A5, ACSB, ACSVL6, FACVL3, FATP5|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP9015c was selected from the Center region of human SLC27A5. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Synonyms||ACSB, ACSVL6, FACVL3, FATP5|
|Function||Acyl-CoA synthetase involved in bile acid metabolism. Proposed to catalyze the first step in the conjugation of C24 bile acids (choloneates) to glycine and taurine before excretion into bile canaliculi by activating them to their CoA thioesters. Seems to activate secondary bile acids entering the liver from the enterohepatic circulation. In vitro, also activates 3-alpha,7- alpha,12-alpha-trihydroxy-5-beta-cholestanate (THCA), the C27 precursor of cholic acid deriving from the de novo synthesis from cholesterol.|
|Cellular Location||Endoplasmic reticulum membrane; Multi-pass membrane protein|
|Tissue Location||Predominantly expressed in liver.|
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Provided below are standard protocols that you may find useful for product applications.
SLC27A5 is an isozyme of very long-chain acyl-CoA synthetase (VLCS). It is capable of activating very long-chain fatty-acids containing 24- and 26-carbons. It is expressed in liver and associated with endoplasmic reticulum but not with peroxisomes. Its primary role is in fatty acid elongation or complex lipid synthesis rather than in degradation.
Watkins,P.A., et.al., Prostaglandins Leukot. Essent. Fatty Acids 60 (5-6), 323-328 (1999)Steinberg,S.J., et.al., J. Biol. Chem. 275 (21), 15605-15608 (2000)
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