|Other Names||Importin-5, Imp5, Importin subunit beta-3, Karyopherin beta-3, Ran-binding protein 5, RanBP5, IPO5, KPNB3, RANBP5|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran- dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (By similarity). Mediates the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5. Binds to a beta-like import receptor binding (BIB) domain of RPL23A. In vitro, mediates nuclear import of H2A, H2B, H3 and H4 histones. Binds to CPEB3 and mediates its nuclear import following neuronal stimulation (By similarity). In case of HIV-1 infection, binds and mediates the nuclear import of HIV-1 Rev.|
|Cellular Location||Cytoplasm. Nucleus. Nucleus, nucleolus. Note=Nucleus; nuclear rim. Found particularly in the nuclear rim and nucleolus|
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Provided below are standard protocols that you may find useful for product applications.
Nucleocytoplasmic transport, a signal- andenergy-dependent process, takes place through nuclear porecomplexes embedded in the nuclear envelope. The import of proteinscontaining a nuclear localization signal (NLS) requires the NLSimport receptor, a heterodimer of importin alpha and beta subunitsalso known as karyopherins. Importin alpha binds the NLS-containingcargo in the cytoplasm and importin beta docks the complex at thecytoplasmic side of the nuclear pore complex. In the presence ofnucleoside triphosphates and the small GTP binding protein Ran, thecomplex moves into the nuclear pore complex and the importinsubunits dissociate. Importin alpha enters the nucleoplasm with itspassenger protein and importin beta remains at the pore.Interactions between importin beta and the FG repeats ofnucleoporins are essential in translocation through the porecomplex. The protein encoded by this gene is a member of theimportin beta family.
Deane, R., et al. Mol. Cell. Biol. 17(9):5087-5096(1997)Yaseen, N.R., et al. Proc. Natl. Acad. Sci. U.S.A. 94(9):4451-4456(1997)Bukrinsky, M.I., et al. Nature 365(6447):666-669(1993)Bukrinsky, M.I., et al. Proc. Natl. Acad. Sci. U.S.A. 89(14):6580-6584(1992)
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