Recombinant Mycoplasma Arginine Deiminase (ADI)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Description | Arginine Deiminase (ADI) is a microbial enzyme from Mycoplasma produced in E.coli. It has high affinity to L-arginine and hydrolyzes L-arginine to citrulline and ammonia. Low concentrations of ADI have been shown to inhibit proliferation in certain cultured cells by arresting the cell cycle in G (1) and/or S phase. Higher concentrations of ADI lead to subsequent apoptosis. Recombinant Mycoplasma Arginine Deiminase is a 46.3 kDa protein consisting of 409 amino acids. |
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BiologicalActivity | Measured by its ability to induce apoptosis in Jurkat cells using a concentration of 100-150 ng/ml. |
Authenticity | Verified by N-terminal and Mass Spectrometry analyses (when applicable). |
Endotoxin | Endotoxin level is <0.1 ng/ µg of protein (<1EU/ µg). |
Protein Content | Verified by UV Spectroscopy and/or SDS-PAGE gel. |
Storage | -20°C |
Precautions | Recombinant Mycoplasma Arginine Deiminase (ADI) is for research use only and not for use in diagnostic or therapeutic procedures. |
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