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Animal-Free Recombinant Human Flt3-Ligand

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Description Flt3-Ligand is a growth factor that regulates proliferation of early hematopoietic cells. Flt3-Ligand binds to cells expressing the tyrosine kinase receptor Flt3. Flt3-Ligand, by itself does not stimulate proliferation of early hematopoietic cells, but synergizes with other CSFs and interleukins to induce growth and differentiation. Unlike SCF, Flt3-Ligand exerts no activity on mast cells. Multiple isoforms of Flt3-Ligand have been identified. The predominant biologically active form is anchored to the cell surface as the extracellular domain of a transmembrane protein (209 a.a.). The membrane-bound isoform can be proteolytically cleaved to generate a biologically active soluble isoform. Recombinant human Flt3-Ligand is a soluble 17.6 kDa protein consisting of 155 amino acid residues.
BiologicalActivity The ED50 was determined by the dose-dependent stimulation of the proliferation of human AML5 cells is ≤ 1.0 ng/ml, corresponding to a specific activity of ≥ 1 x 106 units/mg.
Authenticity Verified by N-terminal and Mass Spectrometry analyses (when applicable).
Endotoxin Endotoxin level is <0.1 ng/ µg of protein (<1EU/ µg).
Protein Content Verified by UV Spectroscopy and/or SDS-PAGE gel.
PrecautionsAnimal-Free Recombinant Human Flt3-Ligand is for research use only and not for use in diagnostic or therapeutic procedures.
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$ 1,131.00
$ 263.00
Cat# PBG10543
(40 western blots)
Availability: 5-7days
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