|Calculated MW||16.5 kDa|
|Other Names||FGF2, HBGF-2, Prostatropin, Basic fibroblast growth factor, Heparin-binding growth factor 2|
|Sequence||MPALPEDGGS GAFPPGHFKD PKRLYCKNGG FFLRIHPDGR VDGVREKSDP HIKLQLQAEE RGVVSIKGVC ANRYLAMKED GRLLASKCVT DECFFFERLE SNNYNTYRSR KYTSWYVALK RTGQYKLGSK TGPGQKAILF LPMSAKS|
|Application Notes||Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions.|
|Storage||-20°C; Lyophilized from a 10 mM Na2PO4, pH 8.0.|
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Provided below are standard protocols that you may find useful for product applications.
Fibroblast Growth Factors, FGFs, are a 22 member family of proteins known to be involved in angiogenesis, wound healing and embryonic development. As a family, they bind to heparin and signal through four receptor tyrosine kinases called, FGFR1, 2, 3 and 4. Although the mechanism remains unclear, FGF-basic 147 (variant of FGF basic 154) is a critical component in keeping embryonic stem cells undifferentiated in cell culture systems. Recombinant human FGF-b 147 (FGF-2) is a non-glycosylated protein, containing 147 amino acids, and having a molecular mass of 16.5 kDa.
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Goshima N.,et al.Nat. Methods 5:1011-1017(2008).
Hillier L.W.,et al.Nature 434:724-731(2005).
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