|Calculated MW||20.1 kDa|
|Other Names||FGF21, Fibroblast growth factor 21, FGFL, UNQ3115/PRO10196|
|Application Notes||Centrifuge the vial prior to opening. Reconstitute in sterile H₂O to a concentration ≥ 100 µg/ml. This solution can then be diluted into other aqueous buffers.|
|Storage||-20°C; Lyophilized from filtered (0.4 mm) solution containing 20 mM Tris, pH 7.4 and 20 mM NaCl|
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Provided below are standard protocols that you may find useful for product applications.
The FGFs are a family of more than 20 small (~17–26 kDa) secreted peptides. The initial characterization of these proteins focused on their ability to stimulate fibroblast proliferation. FGFs modulate cellular activity via at least 5 distinct subfamilies of high-affinity FGF receptors (FGFRs): FGFR-1, -2, -3, and -4, all with intrinsic tyrosine kinase activity and, except for FGFR-4, multiple splice isoforms, and FGFR-5, which lacks an intracellular kinase domain. There is growing evidence that FGFRs can be important for regulation of glucose and lipid homeostasis. FGFR-2 appears to be a key molecule during pancreatic development and FGFR-4 has been implicated in cholesterol metabolism and bile acid synthesis. FGF-21 is preferentially expressed in liver, but an exact knowledge of FGF-21 bioactivity and its mode of action have been lacking to date. FGF-21 is a potent activator of glucose uptake on adipocytes, protects animals from diet-induced obesity when overexpressed in transgenic mice, and lowers blood glucose and triglyceride levels when therapeutically administered to diabetic rodents. Fibroblast Growth Factor-21 Mouse Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 183 amino acids including N-terminal Methionine and having a molecular mass of 20.1 kDa. The amino acid sequence of the recombinant mouse FGF21 is 100% homologous to the amino acid sequence of the mouse FGF21 without signal sequence. The FGF-21 is purified by proprietary chromatographic techniques.
Nishimura T.,et al.Biochim. Biophys. Acta 1492:203-206(2000).
Carninci P.,et al.Science 309:1559-1563(2005).
Kharitonenkov A.,et al.J. Clin. Invest. 115:1627-1635(2005).
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