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IL-17F, murine recombinant protein
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- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
| Primary Accession | Q7TNI7 |
|---|---|
| Calculated MW | 29.8 kDa |
| Gene ID | 257630 |
|---|---|
| Gene Symbol | IL-17F |
| Other Names | IL-17, Interleukin 17, Cytokine ML-1, Interleukin-24 |
| Gene Source | Mouse |
| Source | E. coli |
| Assay&Purity | SDS-PAGE; ≥98% |
| Assay2&Purity2 | HPLC; ≥98% |
| Recombinant | Yes |
| Results | 1.94 ng/ml |
| Target/Specificity | IL-17F |
| Application Notes | Reconstitute in sterile H₂O to a concentration of 0.1 mg/ml (Note: Gently pipetting to dissolve). The solution can then be diluted into other aqueous buffers. |
| Format | Lyophilized protein |
| Storage | -20°C; Lyophilized with no additives |
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Provided below are standard protocols that you may find useful for product applications.
Background
Homodimeric interleukin-17F is a member of the IL-17 family of six homodimeric proteins (IL-17A-17F). Activated CD4+ T cells have also been shown to produce Mouse IL-17F. Activity studies reveal IL-17A to be the most potent, followed by IL-17A/F and IL-17F 100 fold lower than IL-17A. Recombinant Mouse IL-17F produced in E.Coli is a non-glycosylated polypeptide comprised of two monomeric subunit each of mIL-17F. The dimer consists of 266 amino acids, with an approximate molecular weight of 29.8 kDa.
References
Kawaguchi M.,et al.Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases.
Oda N.,et al.Am. J. Respir. Crit. Care Med. 171:12-18(2005).
Gilbert J.M.,et al.Submitted (DEC-2001) to the EMBL/GenBank/DDBJ databases.
Ivanov I.I.,et al.Cell 126:1121-1133(2006).
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