|Calculated MW||52713 Da|
|Application & Usage||The peptide is used for blocking the antibody activity of Smad1. It usually blocks the antibody activity completely in Western blot analysis by incubating the peptide with equal volume of antibody for 30-60 minutes at 37°C.|
|Other Names||Mothers against decapentaplegic homolog 1, MAD homolog 1, Mothers against DPP homolog 1, SMAD family member 1, SMAD 1, Smad1, Smad1, Mad1, Madh1|
|Formulation||50 µg (0.5 mg/ml) in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA and 0.02% thimerosal.|
|Reconstitution & Storage||-20 °C|
|Precautions||Smad1 Blocking Peptide is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD). Has been shown to be also activated by TGF-beta (transforming growth factor) type I receptor kinase in rat intestinal epithelial cells (IEC 4-1). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1. May act synergistically with SMAD4 and YY1 in bone morphogenetic protein (BMP)-mediated cardiac- specific gene expression (By similarity).|
|Cellular Location||Cytoplasm. Nucleus. Note=In the cytoplasm in the absence of ligand Migration to the nucleus when complexed with SMAD4. Colocalizes with LEMD3 at the nucleus inner membrane (By similarity)|
|Tissue Location||Ubiquitous; present in liver, lung, stomach and spleen with lower level in heart, testes and skeletal muscle|
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