|Calculated MW||27.4 kDa|
|Other Names||Neurotrophin-3, NT-3, HDNF, Nerve growth factor 2, NGF-2, Neurotrophic factor|
|Sequence||Recombinant Human ProNT-3 is produced with E.coli expression system. Target protein is expressed with sequence (Asn19-Thr257) of Human (Uniprot #P20783).The sequence of the first five N-terminal amino acids was determined and was found to be Asn-Asn-Met-Asp-Gln.|
|Application Notes||Dissolve in 1x PBS (It is not recommended to reconstitute to a final concentration less than 100 µg/ml.). This can further be diluted to other aqueous buffers.|
|Storage||-20°C; Lyophilized from a 0.2 µm filtered solution of 20 mM PB and 250 mM NaCl, pH 7.2.|
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Proneurotrophin-3 (ProNT-3) is the precursor form of neurotrophin 3. ProNT3 functions via p75NTR and sortilin and induces neuronal apoptosis. On the contrary, mature NT3 interacts with Trk receptors and selectively promotes the survival, growth and differentiation of neurons. As axon retrograde transport is crucial for neurotrophin signaling, Pro-NT3 also plays an important role in the mechanism of retrograde signaling. ProNT3 interacts preferentially with the pan-neurotrophin receptor p75 (p75NTR) and vps10p domain-containing receptor sortilin and induces neuronal apoptosis, whereas mature BDNF selectively binds with high affinity to the TrkB kinase receptor and promotes the survival, growth and differentiation of neurons. As proneurotrophins and mature neurotrophins elicit opposite biological effects, Pro-BDNF cleavage in the neuronal system is regulated in a specific and cell-context dependent manner. Pro-NT3 plays an important role in retrograde signaling mechanism.
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Ota T.,et al.Nat. Genet. 36:40-45(2004).
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