|Calculated MW||~52 kDA|
|Other Names||Brain-derived neurotrophic factor (BDNF) (Abrineurin)|
|Sequence||Recombinant Human precursor form of Brain-derived neurotrophic factor produced in E. coli is a non-glycosylated polypeptide chain containing 2X229 amino acids as a homodimer and having a molecular mass of 52 kDa. The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Pro-Met-Lys-Glu.|
|Application Notes||Dissolve in 1x PBS (It is not recommended to reconstitute to a final concentration less than 100 µg/ml.). This can further be diluted to other aqueous buffers.|
|Storage||-20°C; Lyophilized from a 0.2 µm filtered solution of 20 mM PB and 250 mM NaCl, pH 7.2.|
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Precursor form of Brain-derived neurotrophic factor (Pro-BDNF) interacts preferentially with the pan-neurotrophin receptor p75 (p75NTR) and vps10p domain-containing receptor sortilin and induces neuronal apoptosis, whereas mature BDNF selectively binds with high affinity to the TrkB kinase receptor and promotes the survival, growth and differentiation of neurons. As proneurotrophins and mature neurotrophins elicit opposite biological effects, Pro-BDNF cleavage in the neuronal system is regulated in a specific and cell-context dependent manner. Pro-BDNF plays important role in negative regulation of neurotrophic actions in the brain.
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Liu Q.-R.,et al.Am. J. Med. Genet. B Neuropsychiatr. Genet. 134:93-103(2005).
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