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Endothelial Lipase Blocking Peptide
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
| Primary Accession | Q9Y5X9 |
|---|---|
| Gene ID | 9388 |
| Calculated MW | 56795 Da |
| Gene ID | 9388 |
|---|---|
| Application & Usage | The peptide is used for blocking the antibody activity of Endothelial Lipase. It usually blocks the antibody activity completely in Western blot analysis by incubating the peptide with equal volume of antibody for 30-60 minutes at 37°C |
| Other Names | Endothelial lipase, 3.1.1.3, Endothelial cell-derived lipase, EDL, EL, LIPG |
| Target/Specificity | Endothelial Lipase |
| Formulation | 50 µg (0.5 mg/ml) in phosphate buffered saline (PBS), pH 7.2, containing 0.1% BSA and 0.02% thimerosal. |
| Reconstitution & Storage | -20 °C |
| Background Descriptions | |
| Precautions | Endothelial Lipase Blocking Peptide is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | LIPG |
|---|---|
| Function | Exerts both phospholipase and triglyceride lipase activities (PubMed:12032167, PubMed:10318835, PubMed:10192396). More active as a phospholipase than a triglyceride lipase (PubMed:12032167). Hydrolyzes triglycerides, both with short-chain fatty acyl groups (tributyrin) and long-chain fatty acyl groups (triolein) with similar levels of activity toward both types of substrates (PubMed:12032167). Hydrolyzes high density lipoproteins (HDL) more efficiently than other lipoproteins (PubMed:12032167, PubMed:10192396). |
| Cellular Location | Secreted. |
| Tissue Location | High level of expression in the liver, placenta, lung, thyroid, kidney, testis and in the corpus luteum of the ovary Expressed also in coronary artery endothelial cells, umbilical vein endothelial cells and in hepatocytes and osteosarcoma cell lines. Not detected in heart, brain and muscle. |
Research Areas
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info@abcepta.com, and receive a free "I Love Antibodies" mug.
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
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