|Calculated MW||179145 Da|
|Application & Usage||The peptide is used for blocking the antibody activity of LRP-5/6. It usually blocks the antibody activity completely in Western blot analysis by incubating the peptide with equal volume of antibody for 30-60 minutes at 37°C|
|Other Names||Low-density lipoprotein receptor-related protein 5, LRP-5, LRP5, LR3, LRP7|
|Formulation||50 µg (0.5 mg/ml) in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 0.1% BSA and 0.02% thimerosal.|
|Reconstitution & Storage||-20 °C|
|Precautions||LRP-5/6 Blocking Peptide is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Component of the Wnt-Fzd-LRP5-LRP6 complex that triggers beta-catenin signaling through inducing aggregation of receptor- ligand complexes into ribosome-sized signalsomes. Cell-surface coreceptor of Wnt/beta-catenin signaling, which plays a pivotal role in bone formation. Plays a role in norrin (NDP) signal transduction (PubMed:27228167). The Wnt-induced Fzd/LRP6 coreceptor complex recruits DVL1 polymers to the plasma membrane which, in turn, recruits the AXIN1/GSK3B-complex to the cell surface promoting the formation of signalsomes and inhibiting AXIN1/GSK3-mediated phosphorylation and destruction of beta- catenin. Appears be required for postnatal control of vascular regression in the eye (By similarity). Required for posterior patterning of the epiblast during gastrulation (By similarity).|
|Cellular Location||Membrane; Single-pass type I membrane protein. Endoplasmic reticulum. Note=Chaperoned to the plasma membrane by MESD.|
|Tissue Location||Widely expressed, with the highest level of expression in the liver and in aorta|
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