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UbcH3, human recombinant protein (His-tag)
UbcH3, Ubiquitin conjugating enzyme 3, Ubiquitin conjugating enzyme
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
| Primary Accession | P49427 |
|---|---|
| Concentration | 0.6 |
| Calculated MW | 32.0 kDa |
| Gene ID | 997 |
|---|---|
| Gene Symbol | UB2R1 |
| Other Names | UbcH3, Ubiquitin conjugating enzyme 3, Ubiquitin conjugating enzyme, Ubiquitin-conjugating enzyme E2-32 kDa complementing, Ubiquitin-conjugating enzyme E2-CDC34, Ubiquitin-protein ligase R1, CDC34, UBCH3, UBE2R1 |
| Gene Source | Human |
| Source | E. coli |
| Assay&Purity | SDS-PAGE; ≥95% |
| Assay2&Purity2 | HPLC; |
| Recombinant | Yes |
| Format | Liquid |
| Storage | -80°C; Supplied at a concentration of 0.5 mg/ml in 50 mM HEPES, 50 mM NaCl, pH 8.0, containing 1.0 mM DTT. |
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Provided below are standard protocols that you may find useful for product applications.
Background
UbcH3 is a class II enzyme, homologous to Cdc34 from Saccharomyces cerevisiae, and is important in the control of cell cycle and DNA replication. UbcH3/Cdc34 in association with different E3 complexes, including SCF, has been shown to target many different substrates for ubiquitination and degradation during cell division, signal transduction, and development. UbcH3 substrates that have been characterized include IκB, Wee1, ICERIIγ, p27Xic1. Additionally, substrates such as ß-catenin, p21, and is phosphorylated and ubiquitinylated in vivo.
References
Plon S.E.,et al.Proc. Natl. Acad. Sci. U.S.A. 90:10484-10488(1993).
Kalnine N.,et al.Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
Gonen H.,et al.J. Biol. Chem. 274:14823-14830(1999).
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