|Calculated MW||80745 Da|
|Application & Usage||The peptide is used for blocking the antibody activity of Pak5/Pak7. It usually blocks the antibody activity completely in Western blot analysis by incubating the peptide with equal volume of antibody for 30-60 minutes at 37°C.|
|Other Names||Serine/threonine-protein kinase PAK 7, 188.8.131.52, p21-activated kinase 5, PAK-5, p21-activated kinase 7, PAK-7, PAK7, KIAA1264, PAK5|
|Formulation||50 µg (0.5mg/ml) in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA and 0.02% thimerosal.|
|Reconstitution & Storage||-20 °C|
|Precautions||PAK5/PAK7 Blocking Peptide is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, proliferation or cell survival. Activation by various effectors including growth factor receptors or active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Phosphorylates the proto-oncogene RAF1 and stimulates its kinase activity. Promotes cell survival by phosphorylating the BCL2 antagonist of cell death BAD. Phosphorylates CTNND1, probably to regulate cytoskeletal organization and cell morphology. Keeps microtubules stable through MARK2 inhibition and destabilizes the F-actin network leading to the disappearance of stress fibers and focal adhesions.|
|Cellular Location||Mitochondrion. Cytoplasm. Nucleus. Note=Shuttles between the nucleus and the mitochondria, and mitochondrial localization is essential for the role in cell survival|
|Tissue Location||Predominantly expressed in brain.|
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