|Calculated MW||12-13 kDa|
|Other Names||Cystatin-3, Gamma-trace neuroendocrine basic polypeptide, Post-gamma-globulin|
|Source||Human 293 cells|
|Results||Typically 0.3 to 1.5 µM IC50.|
|Application Notes||Reconstitute in sterile PBS.|
|Storage||-20°C; Lyophilized in PBS|
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Provided below are standard protocols that you may find useful for product applications.
Xeno-free Cystatin C HuXp (Cys-C) is expressed in human 293 cells as a monomer with an apparent molecular mass of 12 to 13 kDa. Native Cys-C in human urine is found in two different forms: one with pI 9.2 and the other with pI 7.8 by elimination of small basic peptides or amino acids from the N-terminal end of protein. Cystatin C has been studied for its role in predicting new onset or deteriorating cardiovascular disease. This cytokine is produced in a serum-free, chemically defined media.
Bobek L.A.,et al.Biochem. J. 278:627-635(1991).
Saitoh E.,et al.Agents Actions 38:340-348(1992).
Dickinson D.P.,et al.Submitted (NOV-2000) to the EMBL/GenBank/DDBJ databases.
Deloukas P.,et al.Nature 414:865-871(2001).
Hawke D.H.,et al.Biochem. Biophys. Res. Commun. 145:1248-1253(1987).
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