|Calculated MW||20.1 kDa (178 aa, 1-158 aa + NT His-Tag)|
|Other Names||Acid phosphatase 1, soluble isoform b, ACP1, HAAP, LMW-PTP, Red cell acid phosphatase 1, Adipocyte acid phosphatase.|
|Sequence||MGSSHHHHHH SSGLVPRGSH MAEQATKSVL FVCLGNICRS PIAEAVFRKL VTDQNISENW VIDSGAVSDW NVGRSPDPRA VSCLRNHGIH TAHKARQITK EDFATFDYIL CMDESNLRDL NRKSNQVKTC KAKIELLGSY DPQKQLIIED PYYGNDSDFE TVYQQCVRCC RAFLEKAH|
|Storage||-80°C; 1 mg/ml solution in 20 mM MES 6.0, 0.1 mM PMSF, 2 mM EDTA and 10% glycerol.|
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Provided below are standard protocols that you may find useful for product applications.
Acid phosphatase 1, also known as ACP1, belongs to the phosphotyrosine protein family. It functions as an acid phosphatase and a protein tyrosine phosphatase (PTPase) and is present in all human tissues, including adipocytes. This enzyme hydrolyzes protein tyrosine phosphate to protein tyrosine and orthophosphate, and also orthophosphoric monoesters to alcohol and orthophosphate.
Dissing J.,et al.J. Biol. Chem. 266:20619-20625(1991).
Dissing J.,et al.Biochim. Biophys. Acta 1121:261-268(1992).
Wo Y.-Y.P.,et al.J. Biol. Chem. 267:10856-10865(1992).
Bryson G.L.M.,et al.Genomics 30:133-140(1995).
Shekels L.L.,et al.Protein Sci. 1:710-721(1992).
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