|Calculated MW||23.8 kDa (207 aa, 1-187 aa + NT His-Tag)|
|Other Names||Dihydrofolate reductase.|
|Sequence||MGSSHHHHHH SSGLVPRGSH MVRPLNCIVA VSQNMGIGKN GDLPWPPLRN EFKYFQRMTT TSSVEGKQNL VIMGRKTWFS IPEKNRPLKD RINIVLSREL KEPPRGAHFL AKSLDDALRL IEQPELASKV DMVWIVGGSS VYQEAMNQPG HLRLFVTRIM QEFESDTFFP EIDLGKYKLL PEYPGVLSEV QEEKGIKYKF EVYEKKD|
|Storage||-80°C; 1 mg/ml solution in 20 mM Tris-HCl buffer (pH 8.0) containing 0.1 M NaCl, 2 mM DTT and 10% glycerol.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Dihydrofolate reductase (DHFR) is a member of the reductase family of enzymes that is ubiquitously expressed in all organisms. DHFR catalyzes the NADPH-dependent reduction of dihydrofolate to tetrahydrofolate, and it is essential for the synthesis of thymidylate, purines and several amino acids. Expression of methotrexate (MTX)-resistant variants of DHFR in normal hematopoietic cells is a potential strategy to permit administration of larger doses of MTX by alleviating drug toxicity in normal cells and tissues that are drug sensitive.
Crouse G.F.,et al.J. Biol. Chem. 257:7887-7897(1982).
Simonsen C.C.,et al.Proc. Natl. Acad. Sci. U.S.A. 80:2495-2499(1983).
McIvor R.S.,et al.Nucleic Acids Res. 18:7025-7032(1990).
Stone D.,et al.J. Biol. Chem. 254:480-488(1979).
McGrogan M.,et al.J. Biol. Chem. 260:2307-2314(1985).
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