Mouse recombinant protein DHFR
Mouse Recombinant DHFR
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Primary Accession | P00375 |
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Concentration | 1 |
Calculated MW | 23.8 kDa (207 aa, 1-187 aa + NT His-Tag) |
Gene ID | 13361 |
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Gene Symbol | DHFR |
Other Names | Dihydrofolate reductase. |
Gene Source | Mouse |
Source | E. Coli |
Assay&Purity | SDS-PAGE; ≥95% |
Assay2&Purity2 | N/A; |
Recombinant | Yes |
Results | >0.2 units/mg |
Sequence | MGSSHHHHHH SSGLVPRGSH MVRPLNCIVA VSQNMGIGKN GDLPWPPLRN EFKYFQRMTT TSSVEGKQNL VIMGRKTWFS IPEKNRPLKD RINIVLSREL KEPPRGAHFL AKSLDDALRL IEQPELASKV DMVWIVGGSS VYQEAMNQPG HLRLFVTRIM QEFESDTFFP EIDLGKYKLL PEYPGVLSEV QEEKGIKYKF EVYEKKD |
Format | Liquid |
Storage | -80°C; 1 mg/ml solution in 20 mM Tris-HCl buffer (pH 8.0) containing 0.1 M NaCl, 2 mM DTT and 10% glycerol. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Dihydrofolate reductase (DHFR) is a member of the reductase family of enzymes that is ubiquitously expressed in all organisms. DHFR catalyzes the NADPH-dependent reduction of dihydrofolate to tetrahydrofolate, and it is essential for the synthesis of thymidylate, purines and several amino acids. Expression of methotrexate (MTX)-resistant variants of DHFR in normal hematopoietic cells is a potential strategy to permit administration of larger doses of MTX by alleviating drug toxicity in normal cells and tissues that are drug sensitive.
References
Crouse G.F.,et al.J. Biol. Chem. 257:7887-7897(1982).
Simonsen C.C.,et al.Proc. Natl. Acad. Sci. U.S.A. 80:2495-2499(1983).
McIvor R.S.,et al.Nucleic Acids Res. 18:7025-7032(1990).
Stone D.,et al.J. Biol. Chem. 254:480-488(1979).
McGrogan M.,et al.J. Biol. Chem. 260:2307-2314(1985).
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