|Calculated MW||23.7 kDa (214 aa, 1-194 aa + NT His Tag)|
|Other Names||Adenylate kinase isoenzyme1, ATP-AMP transphosphorylase 1, Myokinase.|
|Sequence||MGSSHHHHHH SSGLVPRGSH MEEKLKKTKI IFVVGGPGSG KGTQCEKIVQ KYGYTHLSTG DLLRSEVSSG SARGKKLSEI MEKGQLVPLE TVLDMLRDAM VAKVNTSKGF LIDGYPREVQ QGEEFERRIG QPTLLLYVDA GPETMTQRLL KRGETSGRVD DNEETIKKRL ETYYKATEPV IAFYEKRGIV RKVNAEGSVD SVFSQVCTHL DALK|
|Storage||-80°C; 1 mg/ml solution in 20 mM Tris-HCl buffer (pH 7.5) containing 10% glycerol.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
firstname.lastname@example.org, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
AK1 is an enzyme involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of the terminal phosphate group between ATP and AMP. This protein is found in the cytosol of skeletal muscle, brain and erythrocytes. It is a small ubiquitous enzyme which is essential for maintenance and cell growth. Defects in AK1 are the cause of a form of hemolytic anemia. Recombinant human AK1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
von Zabern I.,et al.Eur. J. Biochem. 68:281-290(1976).
Matsuura S.,et al.J. Biol. Chem. 264:10148-10155(1989).
Noma T.,et al.Submitted (DEC-1998) to the EMBL/GenBank/DDBJ databases.
Kalnine N.,et al.Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
Lubec G.,et al.Submitted (DEC-2008) to UniProtKB.
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at email@example.com.