|Calculated MW||23.7 kDa (214 aa, 1-194 aa + NT His Tag)|
|Other Names||Adenylate kinase isoenzyme1, ATP-AMP transphosphorylase 1, Myokinase.|
|Sequence||MGSSHHHHHH SSGLVPRGSH MEEKLKKTKI IFVVGGPGSG KGTQCEKIVQ KYGYTHLSTG DLLRSEVSSG SARGKKLSEI MEKGQLVPLE TVLDMLRDAM VAKVNTSKGF LIDGYPREVQ QGEEFERRIG QPTLLLYVDA GPETMTQRLL KRGETSGRVD DNEETIKKRL ETYYKATEPV IAFYEKRGIV RKVNAEGSVD SVFSQVCTHL DALK|
|Storage||-80°C; 1 mg/ml solution in 20 mM Tris-HCl buffer (pH 7.5) containing 10% glycerol.|
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Provided below are standard protocols that you may find useful for product applications.
AK1 is an enzyme involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of the terminal phosphate group between ATP and AMP. This protein is found in the cytosol of skeletal muscle, brain and erythrocytes. It is a small ubiquitous enzyme which is essential for maintenance and cell growth. Defects in AK1 are the cause of a form of hemolytic anemia. Recombinant human AK1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
von Zabern I.,et al.Eur. J. Biochem. 68:281-290(1976).
Matsuura S.,et al.J. Biol. Chem. 264:10148-10155(1989).
Noma T.,et al.Submitted (DEC-1998) to the EMBL/GenBank/DDBJ databases.
Kalnine N.,et al.Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
Lubec G.,et al.Submitted (DEC-2008) to UniProtKB.
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