|Calculated MW||26.1 kDa|
|Other Names||Protein G|
|Application Notes||Reconstitution in H₂O to a concentration of 5 mg/ml gives a clear solution.|
|Storage||-20°C; Lyophilized with no additives|
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Provided below are standard protocols that you may find useful for product applications.
Protein G binds to the constant region of many species of immunoglobulin G. Protein G can be used to detect, quantify and purify IgG antibodies and antibody/antigen complexes. BioVision’s Recombinant Protein G contains only IgG binding domains. The albumin-binding domain as well as cell wall and cell membrane binding domains have been removed to ensure the maximum specific IgG binding capacity. The 6-His-tag on N-terminus can be used for affinity purification or protein G detection using anti-His-tag antibodies. Protein G binds to all IgG subclasses from human, mouse and rat species. It also binds to total IgG from guinea pig, rabbit, goat, cow, sheep, and horse. The Protein G-Biotin is using purified recombinant protein G biotinylated with ~2 biotins per protein G molecule.
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