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IL-2 Receptor α, Human recombinant protein
soluble IL-2 receptor, TAC-antigen, CD25 antigen
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
| Primary Accession | P31785 |
|---|---|
| Calculated MW | 24.8 kDa (31 kDa on SDSPAGE due to glycosylation) |
| Gene ID | 3561 |
|---|---|
| Gene Symbol | IL2RA |
| Other Names | soluble IL-2 receptor, TAC-antigen, CD25 antigen |
| Gene Source | Human |
| Source | (BTI-Tn-5B1-4) Hi-5 Insect Cells |
| Assay&Purity | SDS-PAGE; ≥98% |
| Assay2&Purity2 | HPLC; |
| Recombinant | Yes |
| Sequence | ELCDDDPPEI PHATFKAMAY KEGTMLNCEC KRGFRRIKSG SLYMLCTGNS SHSSWDNQCQ CTSSATRNTT KQVTPQPEEQ KERKTTEMQS PMQPVDQASL PGHCREPPPW ENEATERIYH FVVGQMVYYQ CVQGYRALHR GPAESVCKMT HGKTRWTQPQ LICTGEMETS QFPGEEKPQA SPEGRPESET SCLVTTTDFQ IQTEMAATME TSIFTTEYQ |
| Target/Specificity | IL-2 Rα |
| Application Notes | Centrifuge the vial prior to opening. Reconstitute in 1x PBS pH 7.2 to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C. |
| Format | Lyophilized powder |
| Storage | -20°C; Sterile filtered through a 0.2 micron filter. Lyophilized from 1x PBS |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
The IL-2 receptor system consists of three non-covalently linked subunits termed IL-2Rα, IL-2Rβ, and IL-2Rγ. The IL-2Rα is a type I transmembrane protein consisting of a 219 amino acid extracellular domain, a 19 amino acid transmembrane domain and a 13 amino acid intracellular domain, which is not involved in the transduction of IL-2 signals. Proteolytic processing of IL-2Rα releases the entire extracellular domain of IL-2Rα thereby generating a 219 amino acid soluble protein called soluble IL-2Rα (sIL-2Rα). The homodimeric form binds IL-2 (KD=10mM) and facilitates IL-2 signaling. The secreted sIL-2Rα is expressed on leukemia cells, lymphoma cells, newly activated T and B cells, as well as on approximately 10% of NK cells. Recombinant human sIL-2Rα is a 24.8 kDa protein containing 219 amino acid residues consisting of only the extracellular domain of IL-2Rα. Due to glycosylation, IL-2Rα has an approximate molecular weight of 31 kDa based on SDSPAGE gel and Mass Spectrometry.
References
Takeshita T.,et al.Science 257:379-382(1992).
Noguchi M.,et al.J. Biol. Chem. 268:13601-13608(1993).
Puck J.M.,et al.Hum. Mol. Genet. 2:1099-1104(1993).
Hayashi A.,et al.Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases.
Ross M.T.,et al.Nature 434:325-337(2005).
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