IL-36β, Human recombinant protein
IL-1F8, IL-1H2, IL-1 eta, IL-1F8
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Primary Accession | Q9UBH0 |
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Calculated MW | 17.3 kDa |
Gene ID | 26525 |
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Gene Symbol | IL36B |
Other Names | IL-1F8, IL-1H2, IL-1 eta, IL-1F8 |
Gene Source | Human |
Source | E.coli |
Assay&Purity | SDS-PAGE; ≥98% |
Assay2&Purity2 | HPLC; |
Recombinant | Yes |
Sequence | MREAAPKSYA IRDSRQMVWV LSGNSLIAAP LSRSIKPVTL HLIACRDTEF SDKEKGNMVY LGIKGKDLCL FCAEIQGKPT LQLKEKNIMD LYVEKKAQKP FLFFHNKEGS TSVFQSVSYP GWFIATSTTS GQPIFLTKER GITNNTNFYL DSVE |
Target/Specificity | IL-36β |
Application Notes | Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C. |
Format | Lyophilized powder |
Storage | -20°C; Sterile filtered through a 0.2 micron filter. Lyophilized from 10 mM Sodium Phosphate, pH 7.5. |
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Provided below are standard protocols that you may find useful for product applications.
Background
The IL-1 family is comprised of 11 structurally related ligands, including recently re-named IL-36α (IL-1F6), β (IL-1F8) and γ (IL-1F9). IL-36β is highly expressed in psoriatic plaques, and at lower levels in various other tissues. IL-36β signals through the IL-1Rrp2 (IL-1R6) receptor, which is primarily expressed on certain dendritic cells. The interaction of the IL-1Rrp2 receptor with IL-36 ligands induces dendritic cell maturation and activation. IL-36β also functions as an agonist of NF-kappaB, and stimulates the production of pro-inflammatory proteins, including IL-6, IL-8, BD-2, and BD-3. Recombinant human IL-36β is a 17.3 kDa protein containing 154 amino acid residues.
References
Smith D.E.,et al.J. Biol. Chem. 275:1169-1175(2000).
Mulero J.J.,et al.Biochem. Biophys. Res. Commun. 263:702-706(1999).
Barton J.L.,et al.Eur. J. Immunol. 30:3299-3308(2000).
Debets R.,et al.J. Immunol. 167:1440-1446(2001).
Busfield S.J.,et al.Genomics 66:213-216(2000).
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