|Calculated MW||8.4 kDa|
|Sequence||TRGSDISKTC CFQYSHKPLP WTWVRSYEFT SNSCSQRAVI FTTKRGKKVC THPRKKWVQK YISLLKTPKQ L|
|Application Notes||Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.|
|Storage||-20°C; Sterile filtered through a 0.2 micron filter. Lyophilized with no additives.|
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Provided below are standard protocols that you may find useful for product applications.
Eotaxin-3 is a CC chemokine that signals through the CCR3 receptor. It is produced by endothelial cells stimulated with IL-4 or IL-13. Eotaxin-3 selectively targets cells expressing CCR3, including eosinophils, basophils, T cells and monocytes. Eotaxin-3 has similar activity to Eotaxin and Eotaxin-2, but the three Eotaxins share only a low degree of sequence homology. Recombinant human Eotaxin-3 is an 8.4 kDa protein containing 71 amino acid residues, including the four highly conserved cysteine residues present in CC chemokines.
Guo R.F.,et al.Genomics 58:313-317(1999).
Shinkai A.,et al.J. Immunol. 163:1602-1610(1999).
Kitaura M.,et al.J. Biol. Chem. 274:27975-27980(1999).
Wang Z.,et al.Submitted (OCT-1998) to the EMBL/GenBank/DDBJ databases.
Berg E.L.,et al.Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases.
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