sFas Receptor, human recombinant protein
soluble Fas receptor (sFasR), TNFRSF6, CD95, Apo I, Fas Antigen
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Primary Accession | P25445 |
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Calculated MW | 17.6 kDa |
Gene ID | 355 |
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Gene Symbol | FASL |
Other Names | soluble Fas receptor (sFasR), TNFRSF6, CD95, Apo I, Fas Antigen |
Gene Source | Human |
Source | E.coli |
Assay&Purity | SDS-PAGE; ≥98% |
Assay2&Purity2 | HPLC; |
Recombinant | Yes |
Sequence | MRLSSKSVNA QVTDINSKGL ELRKTVTTVE TQNLEGLHHD GQFCHKPCPP GERKARDCTV NGDEPDCVPC QEGKEYTDKA HFSSKCRRCR LCDEGHGLEV EINCTRTQNT KCRCKPNFFC NSTVCEHCDP CTKCEHGIIK ECTLTSNTKC KEEGSRS |
Target/Specificity | sFas Receptor |
Application Notes | Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C. |
Format | Lyophilized powder |
Storage | -20°C; Sterile filtered through a 0.2 micron filter. Lyophilized with no additives. |
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Background
Fas and Fas Ligand (FasL) belong to the TNF superfamily and are type I and type II transmembrane proteins, respectively. Binding of FasL to Fas triggers apoptosis in Fas-bearing cells. The mechanism of apoptosis involves recruitment of pro-caspase 8 through an adaptor molecule called FADD followed by processing of the pro-enzyme to active forms. These active caspases then cleave various cellular substrates leading to the eventual cell death. sFasR is capable of inhibiting FasL induced apoptosis by acting as a decoy receptor that serves as a sink for FasL. The full length Fas (receptor) is a 319 amino acid type I transmembrane protein, which contains a 157 amino acid extracellular domain, a 17 amino acid transmembrane domain, and 145 amino acid cytoplasmic domain. Recombinant human soluble Fas (sFas Receptor) is a 157 amino acid polypeptide (17.6 kDa) corresponding to the TNFR homologous cysteine rich extracellular domain Fas.
References
Itoh N.,et al.Cell 66:233-243(1991).
Oehm A.,et al.J. Biol. Chem. 267:10709-10715(1992).
Liu C.,et al.Biochem. J. 310:957-963(1995).
Cascino I.,et al.J. Immunol. 154:2706-2713(1995).
Cascino I.,et al.J. Immunol. 156:13-17(1996).
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