Human CellExp Olfactomedin, human recombinant protein
OLFM4, GC1, GW112, Olfactomedin-4, KIAA4294, OLM4, OlfD, UNQ362, hGC-1, hOLfD
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Primary Accession | Q6UWY5 |
---|---|
Calculated MW | This protein is fused with 6 × His tag at the N-terminus, has a calculated MW of 56 kDa. The predicted N-terminus is Asp 21. DTT-reduced protein migrates as 65-70 kDa due to glycosylation. |
Gene ID | 283298 |
---|---|
Gene Symbol | OLFML1 |
Other Names | OLFM4, GC1, GW112, Olfactomedin-4, KIAA4294, OLM4, OlfD, UNQ362, hGC-1, hOLfD |
Gene Source | Human |
Source | HEK 293 cells |
Assay&Purity | SDS-PAGE; ≥95% |
Assay2&Purity2 | HPLC; |
Recombinant | Yes |
Target/Specificity | Olfactomedin |
Application Notes | Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C. |
Format | Lyophilized powder |
Storage | -20°C; Lyophilized from 0.22 µm filtered solution in PBS. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
info@abcepta.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
Background
Olfactomedin-4 (OLM-4) also known as OLFM4, antiapoptotic protein GW112, G-CSF-stimulated clone 1 protein (hGC-1), hOLfD, is a secreted protein which contains one olfactomedin-like domain. OLFM4 is expressed during myeloid lineage development. Much higher expression in bone marrow neutrophils than in peripheral blood neutrophils (at protein level). Strongly expressed in the prostate, small intestine and colon and moderately expressed in the bone marrow and stomach. Overexpressed in some pancreatic cancer tissues. OLFM4 may promote proliferation of pancreatic cancer cells by favoring the transition from the S to G2/M phase. In myeloid leukemic cell lines, inhibits cell growth and induces cell differentiation and apoptosis. OLFM4 may play a role in the inhibition of EIF4EBP1 phosphorylation/deactivation. Facilitates cell adhesion, most probably through interaction with cell surface lectins and cadherin. The human OLFM4 is also thought to be a useful marker for early myeloid development.
References
Clark H.F.,et al.Genome Res. 13:2265-2270(2003).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Taylor T.D.,et al.Nature 440:497-500(2006).
Zhang Z.,et al.Protein Sci. 13:2819-2824(2004).
Wan B.,et al.FEBS Lett. 582:3185-3192(2008).
If you have used an Abcepta product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at tech@abcepta.com.