Human CellExp ADIPOQ /Adiponectin /ACRP30, human recombinant protein
ADIPOQ, Adiponectin, ACDC, ACRP30, APM1, GBP28
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Primary Accession | Q15848 |
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Calculated MW | The protein is fused with 6×His tag at the C-terminus, has a calculated MW of 25.4 kDa. The predicted N-terminus is Glu 19. DTT-reduced Protein migrates as 28-33 kDa due to glycosylation. |
Gene ID | 9370 |
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Gene Symbol | ADIPOQ |
Other Names | ADIPOQ, Adiponectin, ACDC, ACRP30, APM1, GBP28 |
Gene Source | Human |
Source | HEK 293 cells |
Assay&Purity | SDS-PAGE; ≥92% |
Assay2&Purity2 | HPLC; |
Recombinant | Yes |
Target/Specificity | Adiponectin |
Application Notes | Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C. |
Format | Lyophilized powder |
Storage | -20°C; Lyophilized from 0.22 µm filtered solution in PBS. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization. |
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Background
Adiponectin also referred to as GBP-28, APM1, ADIPOQ, ACDC and Acrp30, is a secreted protein which contains one C1q domain (commonly called the globular domain) and one collagen-like domain. Adiponectin / ADIPOQ is synthesized exclusively by adipocytes and secreted into plasma. ADIPOQ is important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Adiponectin antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Acrp30 Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway and may play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW.
References
Maeda K.,et al.Biochem. Biophys. Res. Commun. 221:286-289(1996).
Saito K.,et al.Gene 229:67-73(1999).
Schaeffler A.,et al.Biochem. Biophys. Res. Commun. 260:416-425(1999).
Zhang Z.,et al.Protein Sci. 13:2819-2824(2004).
Nakano Y.,et al.J. Biochem. 120:803-812(1996).
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