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Human CellExp DKK3, human recombinant protein
DKK3, REIC, RIG
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
| Primary Accession | Q9UBP4 |
|---|---|
| Calculated MW | This protein is fused with 6×His tag at the C-terminus, has a calculated MW of 37.6 kDa. The predicted N-terminus is Pro 23. DTT-reduced Protein migrates as 50-60 kDa due to glycosylation. |
| Gene ID | 27122 |
|---|---|
| Gene Symbol | DKK3 |
| Other Names | DKK3, REIC, RIG |
| Gene Source | Human |
| Source | HEK 293 cells |
| Assay&Purity | SDS-PAGE; ≥95% |
| Assay2&Purity2 | HPLC; |
| Recombinant | Yes |
| Target/Specificity | DKK3 |
| Application Notes | Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C. |
| Format | Lyophilized powder |
| Storage | -20°C; Lyophilized from 0.22 µm filtered solution in PBS. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Members of the Dickkopf-related protein family (DKK-1, -2, -3, and -4) are secreted proteins with two cysteine-rich domains separated by a linker region. And DKK3 has been proposed as tumor suppressor gene and a marker for tumor blood vessels. DKK3 is the only DKK family member abundantly expressed in normal lung, but silenced by promoter hypermethylation in a large fraction of lung cancer cell lines and lung tumors. Downregulation of DKK3 was correlated with tumor progression and expression of nuclear beta-catenin in lung tumors. Ectopic expression of DKK3 in lung cancer cells with DKK3 hypermethylation induced apoptosis and inhibited TCF-4 activity as well as nuclear accumulation of beta-catenin and expression of TCF-4 targets c-Myc and cyclin D1. DKK3 modulates FGF and Activin/Nodal signaling to regulate mesoderm induction during early Xenopus development, was reported.
References
Krupnik V.E.,et al.Gene 238:301-313(1999).
Tsuji T.,et al.Biochem. Biophys. Res. Commun. 268:20-24(2000).
Kobayashi K.,et al.Gene 282:151-158(2002).
Tanaka S.,et al.Submitted (OCT-1999) to the EMBL/GenBank/DDBJ databases.
Tate G.,et al.Submitted (NOV-1999) to the EMBL/GenBank/DDBJ databases.
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