CD300A, human recombinant protein
CD300a molecule, CLM-8, CMRF-35-H9, CMRF-35H, CMRF35-H, CMRF35-H9, CMRF35H, CMRF35H9, IGSF12, IRC1,
|Calculated MW||14.7 kDa (134 aa, 18-128 aa + His Tag), confirmed by MALDI-TOF.|
|Other Names||CD300a molecule, CLM-8, CMRF-35-H9, CMRF-35H, CMRF35-H, CMRF35-H9, CMRF35H, CMRF35H9, IGSF12, IRC1, IRC1/IRC2, IRC2, IRp60|
|Sequence||MGSSHHHHHH SSGLVPRGSH MGSLSKCRTV AGPVGGSLSV QCPYEKEHRT LNKYWCRPPQ IFLCDKIVET KGSAGKRNGR VSIRDSPANL SFTVTLENLT EEDAGTYWCG VDTPWLRDFH DPVVEVEVSV FPAS|
|Storage||-80°C; 0.5 mg/ml in 20 mM Tris-HCl buffer (pH 8.0) containing 0.15 M NaCl, 10% glycerol and 1 mM DTT.|
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Provided below are standard protocols that you may find useful for product applications.
CD300A belongs to the CD300 family and contains 1 Ig-like V-type (immunoglobulin-like) domain. The protein is expressed not only by natural killer (NK) cells but also by T-cell subsets, B-cells, dendritic cells, mast cells, granulocytes and monocytes. CD300A is inhibitory receptor which may contribute to the down-regulation of cytolytic activity in natural killer (NK) cells, and to the down-regulation of mast cell degranulation. Recombinant human CD300A protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Cantoni C.,et al.Eur. J. Immunol. 29:3148-3159(1999).
Cantoni C.,et al.Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases.
Green B.J.,et al.Int. Immunol. 10:891-899(1998).
Clark G.J.,et al.Tissue Antigens 55:101-109(2000).
Zhang Q.H.,et al.Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases.
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