CD83, human recombinant protein
CD83 antigen isoform b precursor, BL11, HB15
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Primary Accession | Q01151 |
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Concentration | 0.25 |
Calculated MW | 16 kDa (148 aa, 20-144 aa + His Tag), confirmed by MALDI-TOF. |
Gene ID | 9308 |
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Gene Symbol | CD83 |
Other Names | CD83 antigen isoform b precursor, BL11, HB15 |
Gene Source | Human |
Source | E. coli |
Assay&Purity | SDS-PAGE; ≥90% |
Assay2&Purity2 | N/A; |
Recombinant | Yes |
Sequence | MGSSHHHHHH SSGLVPRGSH MGSTPEVKVA CSEDVDLPCT APWDPQVPYT VSWVKLLEGG EERMETPQED HLRGQHYHQK GQNGSFDAPN ERPYSLKIRN TTSCNSGTYR CTLQDPDGQR NLSGKVILRV TGCPAQRKEE TFKKYRAE |
Target/Specificity | CD83 |
Format | Liquid |
Storage | -80°C; 0.25 mg/ml in 20 mM Tris-HCl buffer (pH 8.0) containing 0.15 M NaCl and 30% glycerol. |
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Background
CD83 is a single-pass type I membrane protein and member of the immunoglobulin superfamily of receptors. The protein may be involved in the regulation of antigen presentation. A soluble form of this protein can bind to dendritic cells and inhibit their maturation. Three transcript variants encoding different isoforms have been found for this gene. Recombinant human CD83 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
References
Zhou L.-J.,et al.J. Immunol. 149:735-742(1992).
Kozlow E.J.,et al.Blood 81:454-461(1993).
Ebert L.,et al.Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
Mungall A.J.,et al.Nature 425:805-811(2003).
Zhang Z.,et al.Protein Sci. 13:2819-2824(2004).
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