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Catalase, human recombinant protein

CAT

     
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Product info
Primary Accession P04040
Concentration 1
Calculated MW 61.9 kDa (547 aa, 1-527 aa + His Tag).
Additional Info
Gene ID 847
Gene Symbol CAT
Other Names CAT
Gene Source Human
Source E. coli
Assay&Purity SDS-PAGE; ≥90%
Assay2&Purity2 N/A;
Recombinant Yes
Sequence MGSSHHHHHH SSGLVPRGSH MADSRDPASD QMQHWKEQRA AQKADVLTTG AGNPVGDKLN VITVGPRGPL LVQDVVFTDE MAHFDRERIP ERVVHAKGAG AFGYFEVTHD ITKYSKAKVF EHIGKKTPIA VRFSTVAGES GSADTVRDPR GFAVKFYTED GNWDLVGNNT PIFFIRDPIL FPSFIHSQKR NPQTHLKDPD MVWDFWSLRP ESLHQVSFLF SDRGIPDGHR HMNGYGSHTF KLVNANGEAV YCKFHYKTDQ GIKNLSVEDA ARLSQEDPDY GIRDLFNAIA TGKYPSWTFY IQVMTFNQAE TFPFNPFDLT KVWPHKDYPL IPVGKLVLNR NPVNYFAEVE QIAFDPSNMP PGIEASPDKM LQGRLFAYPD THRHRLGPNY LHIPVNCPYR ARVANYQRDG PMCMQDNQGG APNYYPNSFG APEQQPSALE HSIQYSGEVR RFNTANDDNV TQVRAFYVNV LNEEQRKRLC ENIAGHLKDA QIFIQKKAVK NFTEVHPDYG SHIQALLDKY NAEKPKNAIH TFVQSGSHLA AREKANL
Target/Specificity Catalase
Format Liquid
Storage -80°C; 1 mg/ml in 20 mM Tris-HCl buffer (pH 8.0) containing 0.15 M NaCl and 10% glycerol.
Citations (0)

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Background

CAT is a key antioxidant enzyme in the bodies’ defense against oxidative stress. Also, it is a heme enzyme that is present in the peroxisome of nearly all aerobic cells. CAT converts the reactive oxygen species hydrogen peroxide to water and oxygen and thereby mitigates the toxic effects of hydrogen peroxide. Recombinant human CAT protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

References

Quan F.,et al.Nucleic Acids Res. 14:5321-5335(1986).
Bell G.I.,et al.Nucleic Acids Res. 14:5561-5562(1986).
Jin L.H.,et al.Free Radic. Biol. Med. 31:1509-1519(2001).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Taylor T.D.,et al.Nature 440:497-500(2006).

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$ 295.00
Cat# PBV10985r
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