Cytidine deaminase, human recombinant protein
CDA, CDD, Cytidine aminohydrolase, Cytosine nucleoside deaminase
|Calculated MW||18.3 kDa (166 aa, 1-146 aa + His Tag).|
|Other Names||CDA, CDD, Cytidine aminohydrolase, Cytosine nucleoside deaminase|
|Sequence||MGSSHHHHHH SSGLVPRGSH MAQKRPACTL KPECVQQLLV CSQEAKQSAY CPYSHFPVGA ALLTQEGRIF KGCNIENACY PLGICAERTA IQKAVSEGYK DFRAIAIASD MQDDFISPCG ACRQVMREFG TNWPVYMTKP DGTYIVMTVQ ELLPSSFGPE DLQKTQ|
|Storage||-80°C; 0.5 mg/ml in 20 mM Tris-HCl buffer (pH 8.0) containing 100 mM NaCl, 1 mM DTT, 2 mM EDTA and 40% glycerol.|
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Provided below are standard protocols that you may find useful for product applications.
CDA (Cytidine deaminase) is an enzyme that scavenges exogenous and endogenous cytidine and 2'-deoxycytidine for UMP synthesis. This protein is one of several deaminases responsible for maintaining the cellular pyrimidine pool. The protein also catalyzes the deamination of chemotherapeutic cytosine nucleoside analogs such as Ara-C and 5-azacytidine, which results in the loss of their cytotoxic and antitumor function. It can form homotetramers and is mainly expressed in granulocytes. Recombinant human CDA protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
Laliberte J.,et al.Cancer Res. 54:5401-5407(1994).
Demontis S.,et al.Biochim. Biophys. Acta 1443:323-333(1998).
Gran C.,et al.Blood 91:4127-4135(1998).
Gregory S.G.,et al.Nature 441:315-321(2006).
Kuhn K.,et al.Biochem. Biophys. Res. Commun. 190:1-7(1993).
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