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Human CellExp CD244 / 2B4 / SLAMF4, human recombinant protein
CD244, 2B4, SLAMF4, NKR2B4, NAIL, h2B4
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
| Primary Accession | Q9BZW8 |
|---|---|
| Calculated MW | This protein is fused with polyhistidine tag at the C-terminus, and has a calculated MW of 23.1 kDa. The predicted N-terminus is Cys 22. DTT-reduced Protein migrates as 40-50 kDa due to glycosylation. |
| Gene ID | 51744 |
|---|---|
| Gene Symbol | CD244 |
| Other Names | CD244, 2B4, SLAMF4, NKR2B4, NAIL, h2B4 |
| Gene Source | Human |
| Source | HEK293 cells |
| Assay&Purity | SDS-PAGE; ≥95% |
| Assay2&Purity2 | N/A; |
| Recombinant | Yes |
| Results | Measured by its binding ability in a functional ELISA. Immobilized rh CD244 / 2B4 at 2 µg/ml (100 µl/well) can bind human CD48. The EC50 of human CD48 is 0.39 µg/ml. |
| Target/Specificity | CD244/2B4/SLAMF4 |
| Application Notes | Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C. |
| Format | Lyophilized |
| Storage | -20°C; Lyophilized from 0.22 µm filtered solution in PBS, pH7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
Natural killer cell receptor 2B4 is also known as NK cell type I receptor protein 2B4 (NKR2B4 or h2B4), SLAM family member 4 (SLAMF4), Signaling lymphocytic activation molecule 4, CD antigen CD244. NKR2B4 / CD244 contain two Ig-like (immunoglobulin-like) domains. CD244 is expressed in spleen, PBL, followed by lung, liver, testis and small intestine. CD244 interacts with CD48. Following phosphorylation, CD244 is able to recruit PTPN11/SHP-2 and SH2D1A/SAP. SLAMF4 modulate other receptor-ligand interactions to enhance leukocyte activation. CD244/2B4 is the only heterophilic receptor of SLAM family.
References
Nakajima H.,et al.Eur. J. Immunol. 29:1676-1683(1999).
Kubin M.Z.,et al.Eur. J. Immunol. 29:3466-3477(1999).
Tangye S.G.,et al.J. Immunol. 162:6981-6985(1999).
Boles K.S.,et al.Tissue Antigens 54:27-34(1999).
Kumaresan P.R.,et al.Immunogenetics 51:987-992(2000).
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